Structural studies by electron tomography: from cells to molecules
▪ Abstract Electron tomography (ET) is uniquely suited to obtain three-dimensional
reconstructions of pleomorphic structures, such as cells, organelles or supramolecular …
reconstructions of pleomorphic structures, such as cells, organelles or supramolecular …
Electron tomography: a three‐dimensional analytic tool for hard and soft materials research
Three‐dimensional (3D) structural analysis is essential to understand the relationship
between the structure and function of an object. Many analytical techniques, such as X‐ray …
between the structure and function of an object. Many analytical techniques, such as X‐ray …
EVpedia: an integrated database of high‐throughput data for systemic analyses of extracellular vesicles
Secretion of extracellular vesicles is a general cellular activity that spans the range from
simple unicellular organisms (eg archaea; Gram‐positive and Gram‐negative bacteria) to …
simple unicellular organisms (eg archaea; Gram‐positive and Gram‐negative bacteria) to …
Optimal determination of particle orientation, absolute hand, and contrast loss in single-particle electron cryomicroscopy
PB Rosenthal, R Henderson - Journal of molecular biology, 2003 - Elsevier
A computational procedure is described for assigning the absolute hand of the structure of a
protein or assembly determined by single-particle electron microscopy. The procedure …
protein or assembly determined by single-particle electron microscopy. The procedure …
Gram‐positive bacteria produce membrane vesicles: Proteomics‐based characterization of Staphylococcus aureus‐derived membrane vesicles
Although archaea, Gram‐negative bacteria, and mammalian cells constitutively secrete
membrane vesicles (MVs) as a mechanism for cell‐free intercellular communication, this …
membrane vesicles (MVs) as a mechanism for cell‐free intercellular communication, this …
Macromolecular architecture in eukaryotic cells visualized by cryoelectron tomography
Electron tomography of vitrified cells is a noninvasive three-dimensional imaging technique
that opens up new vistas for exploring the supramolecular organization of the cytoplasm. We …
that opens up new vistas for exploring the supramolecular organization of the cytoplasm. We …
Biological imaging by soft x-ray diffraction microscopy
We have used the method of x-ray diffraction microscopy to image the complex-valued exit
wave of an intact and unstained yeast cell. The images of the freeze-dried cell, obtained by …
wave of an intact and unstained yeast cell. The images of the freeze-dried cell, obtained by …
Cryo transmission electron microscopy of liposomes and related structures
Cryo-transmission electron microscopy, c-TEM, has during the last 10 years contributed
significantly to the understanding of the numerous, and often complex, structures formed by …
significantly to the understanding of the numerous, and often complex, structures formed by …
From words to literature in structural proteomics
Technical advances on several frontiers have expanded the applicability of existing methods
in structural biology and helped close the resolution gaps between them. As a result, we are …
in structural biology and helped close the resolution gaps between them. As a result, we are …
[HTML][HTML] The energy dependence of contrast and damage in electron cryomicroscopy of biological molecules
We have measured the dependence on electron energy of elastic and inelastic scattering
cross-sections from carbon, over the energy range that includes 100 keV to 300 keV. We …
cross-sections from carbon, over the energy range that includes 100 keV to 300 keV. We …