Single-cell RNA sequencing is at the forefront of high-resolution phenotyping experiments
for complex samples. Although this methodology requires specialized equipment and …

RNA sequencing using next-generation sequencing technologies (NGS) is currently the
standard approach for gene expression profiling, particularly for large-scale high-throughput …

High-throughput single-cell RNA-sequencing (scRNA-seq) methodologies enable
characterization of complex biological samples by increasing the number of cells that can be …

Sphingolipids are structural membrane components and important eukaryotic signaling
molecules. Sphingolipids regulate inflammation and immunity and were recently identified …

RNA sequencing (RNA-seq) is a sensitive and accurate method for quantifying gene
expression. Small samples or those whose RNA is degraded, such as formalin-fixed paraffin …

The recent advent of ribosome profiling—sequencing of short ribosome-bound fragments of
mRNA—has offered an unprecedented opportunity to interrogate the sequence features …

The adaptive immune system's capability to protect the body requires a highly diverse
lymphocyte antigen receptor repertoire. However, the influence of individual genetic and …

Single-cell RNA sequencing has revealed extensive cellular heterogeneity within many
organisms, but few methods have been developed for microbial clonal populations. The …

Single-cell RNA-Seq (scRNA-Seq) has led to an unprecedented understanding of gene
expression and regulation in individual cells. Many scRNA-Seq approaches rely upon the …

Next-generation RNA sequencing (RNA-seq) has revolutionized our ability to analyze
transcriptomes. Current RNA-seq methods are highly reproducible, but each has biases …