Yeasts have been used for thousands of years to make fermented foods and beverages,
such as beer, wine, sake, and bread. However, the choice for a particular yeast strain or …

C. elegans has distinguishing characteristics that predestine this organism for the
visualization of gene expression patterns using the reporter genes green fluorescent protein …

A new type of gel electrophoresis separates DNA molecules up to 2000 kb with resolutions
exceeding the logarithmic molecular weight dependence of conventional electrophoresis …

The Sc2. 0 project is building a eukaryotic synthetic genome from scratch. A major milestone
has been achieved with all individual Sc2. 0 chromosomes assembled. Here, we describe …

Yeast strains were constructed in which a single telo mere could be eliminated from the end
of a dispensable chromosome. In wild-type cells, elimination of a telomere caused a RADS …

A screen to detect yeast mutants that frequently lost expression of subtelomeric genes
identified two mutations in PIF1, a gene known to encode a 5'to 3'DNA helicase. The loss of …

Fifty-five-kilobase long artificial chromosomes containing cloned genes, replicators,
centromeres and telomeres have been constructed in yeast. These molecules have many of …

To identify gene products that function stoichiometrically in mitotic chromosome
transmission, genes were cloned on high copy number plasmids and transformed into yeast …

A colony color assay that measures chromosome stability is described and is used to study
several parameters affecting the mitotic maintenance of yeast chromosomes, including ARS …

The Saccharomyces cerevisiae genome encodes seven homologues of the mammalian
oxysterol-binding protein (OSBP), a protein implicated in lipid trafficking and sterol …