Extend the survival of human sperm in vitro in non-freezing conditions: damage mechanisms, preservation technologies, and clinical applications

Q Cheng, L Li, M Jiang, B Liu, Y Xian, S Liu, X Liu… - Cells, 2022 - mdpi.com
Preservation of human spermatozoa in vitro at normothermia or hypothermia maintaining
their functions and fertility for several days plays a significant role in reproductive biology …

Human sperm vitrification: A scientific report

M Schulz, J Risopatrón, P Uribe, E Isachenko… - …, 2020 - Wiley Online Library
Background The sperm vitrification developed by this group is based on the ultrarapid
freezing of a vitrification solution composed of a non‐permeable cryoprotectant (saccharides …

Elamipretide as a potential candidate for relieving cryodamage to human spermatozoa during cryopreservation

H Bai, Y Zhang, S Tian, R Hu, Y Liang, J Gao, Y Wang… - Cryobiology, 2020 - Elsevier
As a technique widely used in assisted reproduction, human spermatozoa cryopreservation
makes it possible to conserve functional sperm for a long time, but the impact of cryodamage …

Multiparameter flow cytometry assay for analysis of nitrosative stress status in human spermatozoa

P Uribe, J Meriño, E Manquemilla, C Villagrán… - Cytometry Part …, 2020 - Wiley Online Library
Male infertility is an increasing health problem, and oxidative/nitrosative stress plays an
important role in the etiology of this condition. Nitrosative stress due to excessive levels of …

[HTML][HTML] First pregnancies in jennies with vitrified donkey semen using a new warming method

M Diaz-Jimenez, A Rota, J Dorado, C Consuegra… - Animal, 2021 - Elsevier
Sperm vitrification has been recently developed, but fertility trials have not been performed
yet in equine species. In this study, a new warming technique for vitrified donkey semen was …

Dynamic assessment of human sperm DNA damage III: The effect of sperm freezing techniques

E Tvrdá, J Gosálvez, F Arroyo, P Sánchez… - Cell and Tissue …, 2021 - Springer
Among the currently available strategies for sperm freezing, vitrification may be considered
as the leading alternative to conventional cryopreservation. Nevertheless, a direct …

Medical Thermology 2017-a computer-assisted literature survey.

K Ammer - Thermology International, 2018 - search.ebscohost.com
Methods A search in Scopus with the terms" thermology" OR" thermography" OR" infrared
thermal imaging" OR" infrared imaging" OR" thermal imaging" OR" thermometry" OR" …

Impact of time between repeated sperm freezing cycles on sperm quality

B Zaghi, S Barda, SE Kleiman, R Hauser - Reproductive Biology, 2020 - Elsevier
Refreezing of sperm samples would provide the possibility of performing more cycles of
fertility treatments. Although the effect of repeated cycles of freezing on sperm quality was …

Protective effect of the superoxide dismutase mimetic MnTBAP during sperm vitrification process

P Uribe, C Cárcamo, E Navarro, J Sepúlveda… - Andrologia, 2020 - Wiley Online Library
Sperm cryopreservation is widely used in assisted reproduction and male infertility therapy;
however, it induces oxidative stress affecting sperm quality. This work evaluated the effect of …

Cryo-banking of human spermatozoa by aseptic cryoprotectants-free vitrification in liquid air: Positive effect of elevated warming temperature

M Diaz-Jimenez, M Wang, W Wang, E Isachenko… - Cell and Tissue …, 2022 - Springer
Cryoprotectant-free vitrification is a common method for spermatozoa cryopreservation by
direct plunging into liquid nitrogen. However, the commercial liquid nitrogen could be …