Scalable sequence–function studies have enabled the systematic analysis and cataloging of
hundreds of thousands of coding and noncoding genetic variants in the human genome …

Assigning functions to genes and learning how to control their expression are part of the
foundation of cell biology and therapeutic development. An efficient and unbiased method to …

Candidate cis-regulatory elements (cCREs) in microglia demonstrate the most substantial
enrichment for Alzheimer's disease (AD) heritability compared to other brain cell types …

Abstract We present Light-Seq, an approach for multiplexed spatial indexing of intact
biological samples using light-directed DNA barcoding in fixed cells and tissues followed by …

The CRISPR genome editing technology has revolutionized the way gene function is
studied. Genome editing can be achieved in single genes or for thousands of genes …

Linking variants from genome-wide association studies (GWAS) to underlying mechanisms
of disease remains a challenge,–. For some diseases, a successful strategy has been to …

Single-cell RNA sequencing (scRNA-seq) and single-nucleus RNA-seq (snRNA-seq) allow
transcriptomic profiling of thousands of cells from a renal biopsy specimen at a single-cell …

Sequencing costs currently prohibit the application of single-cell mRNA-seq to many
biological and clinical analyses. Targeted single-cell mRNA-sequencing reduces …

Long noncoding RNAs (lncRNAs) are a class of RNA molecules exceeding 200 nucleotides
in length that lack long open-reading frames. Transcribed predominantly by RNA …

CRISPR-based gene activation (CRISPRa) is a promising therapeutic approach for gene
therapy, upregulating gene expression by targeting promoters or enhancers in a tissue/cell …