Integration of microfluidics in animal in vitro embryo production
MB Wheeler, M Rubessa - MHR: Basic science of reproductive …, 2017 - academic.oup.com
The in vitro production of livestock embryos is central to several areas of animal
biotechnology. Further, the use of in vitro embryo manipulation is expanding as new …
biotechnology. Further, the use of in vitro embryo manipulation is expanding as new …
Reproductive outcomes predicted by phase imaging with computational specificity of spermatozoon ultrastructure
The ability to evaluate sperm at the microscopic level, at high-throughput, would be useful
for assisted reproductive technologies (ARTs), as it can allow specific selection of sperm …
for assisted reproductive technologies (ARTs), as it can allow specific selection of sperm …
On the estimation of heat-intensity and heat-duration effects in time series models of temperature-related mortality in Stockholm, Sweden
Background We examine the effect of heat waves on mortality, over and above what would
be predicted on the basis of temperature alone. Methods Present modeling approaches may …
be predicted on the basis of temperature alone. Methods Present modeling approaches may …
Carnitine supplementation decreases capacitation-like changes of frozen-thawed buffalo spermatozoa
The aim of this study was to evaluate the effect of carnitine supplementation of semen
extender on fertility parameters of frozen-thawed buffalo sperm. Buffalo semen was …
extender on fertility parameters of frozen-thawed buffalo sperm. Buffalo semen was …
Oxidative stress differentially impacts male and female bovine embryos depending on the culture medium and the stress condition
M Dallemagne, E Ghys, C De Schrevel, A Mwema… - Theriogenology, 2018 - Elsevier
Male and female embryos are known to differ for their metabolism and response to
environmental factors very early in development. The present study aimed to evaluate the …
environmental factors very early in development. The present study aimed to evaluate the …
SLIM microscopy allows for visualization of DNA-containing liposomes designed for sperm-mediated gene transfer in cattle
Naked DNA has been shown to bind naturally to the sperm, a method called sperm-
mediated gene transfer (SMGT). Based on these observations, we examined the efficiency …
mediated gene transfer (SMGT). Based on these observations, we examined the efficiency …
How to beat the bull: Lycopene as a tool to improve in vitro fertilization efficiency in bulls with high polyspermy
A Fernández-Montoro, D Angel-Velez, B Cava-Cami… - Reproductive …, 2024 - Elsevier
High levels of reactive oxygen species (ROS) derived from in vitro conditions compromise
oocyte quality and subsequent polyspermy prevention by the zona and membrane block …
oocyte quality and subsequent polyspermy prevention by the zona and membrane block …
Effect of energy source during culture on in vitro embryo development, resistance to cryopreservation and sex ratio
M Rubessa, L Boccia, G Campanile, V Longobardi… - Theriogenology, 2011 - Elsevier
The aim of this work was to evaluate whether minimizing the glucose concentration during
culture or replacing the hexose with other energy substrates and/or embryotrophic …
culture or replacing the hexose with other energy substrates and/or embryotrophic …
Morphometric analysis of sperm used for IVP by three different separation methods with spatial light interference microscopy
The goal of this study was to characterize sperm populations resulting from three different
methods of sperm selection used for bovine in vitro fertilization. We compared sperm …
methods of sperm selection used for bovine in vitro fertilization. We compared sperm …
Non-invasive nuclear magnetic resonance analysis of male and female embryo metabolites during in vitro embryo culture
Introduction In the past 20+ years, several studies of bovine embryo production showed how
the ratio of male to female embryos changes if embryos are made in vivo or in vitro. It is …
the ratio of male to female embryos changes if embryos are made in vivo or in vitro. It is …