Current technology enables the production of highly specific genome modifications with
excellent efficiency and specificity. Key to this capability are targetable DNA cleavage …

Laboratory animal models play an important role in the study of human diseases. Using
appropriate animals is critical not only for basic research but also for the development of …

The common carp (Cyprinus carpio) as one of the most important aquaculture fishes
produces over 3 million metric tones annually, approximately 10% the annual production of …

Programmable nucleases allow defined alterations in the genome with ease-of-use,
efficiency, and specificity. Their availability has led to accurate and widespread genome …

Livestock is an essential life commodity in modern agriculture involving breeding and
maintenance. The farming practices have evolved mainly over the last century for …

Cell therapy is an accessible method for curing damaged organs or tissues. Yet, this
approach is limited by the delivery efficiency of cell suspension injection. Over recent years …

Inbred mini-pigs are ideal organ donors for future human xenotransplantations because of
their clear genetic background, high homozygosity, and high inbreeding endurance. In this …

Pigs share many physiological, biochemical, and anatomical similarities with humans and
have emerged as valuable large animal models for biomedical research. Considering the …

Cataracts are the leading cause of vision loss in the world, although surgical treatment can
restore vision in cataract patients. Until now, there have been no adequate animal models …

The prokaryotic clustered regularly interspaced short palindromic repeat (CRISPR)-
associated system (Cas) is a simple, robust and efficient technique for gene targeting in …