Recombinant Chinese hamster ovary cells (rCHO) cells have been the most commonly used
mammalian host for large-scale commercial production of therapeutic proteins. Recent …

Virus-like particles (VLPs) are multiprotein structures that mimic the organization and
conformation of authentic native viruses but lack the viral genome, potentially yielding safer …

Apoptosis is a form of programmed and controlled cell death that accounts for the majority of
cellular death in bioprocesses. Cell death affects culture longevity and product quality; it is …

Background Nanometer silicon dioxide (nano-SiO 2) has a wide variety of applications in
material sciences, engineering and medicine; however, the potential cell biological and …

Human mesenchymal stem/stromal cells (hMSCs) present a key therapeutic cellular
intervention for use in cell and gene therapy (CGT) applications due to their …

The effects on human mesenchymal stem cell growth of choosing either of two spinner flask
impeller geometries, two microcarrier concentrations and two cell concentrations (seeding …

Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) continues to be one of the
most versatile and widely used techniques to study the proteome of a biological system. In …

The recruitment of ribosomes to eukaryotic cellular mRNAs requires the activity of two
prototypic RNA helicases, eukaryotic initiation factor (eIF) 4AI and eIF4AII. The eIF4A …

Increasing demand for large-scale synthesis of in vitro transcribed (IVT) mRNA is being
driven by the increasing use of mRNA for transient gene expression in cell engineering and …

Most of the clinical approved protein-based drugs or under clinical trials have a profound
impact on the treatment of critical diseases. The mammalian eukaryotic cells culture …