CHO cells in biotechnology for production of recombinant proteins: current state and further potential
Recombinant Chinese hamster ovary cells (rCHO) cells have been the most commonly used
mammalian host for large-scale commercial production of therapeutic proteins. Recent …
mammalian host for large-scale commercial production of therapeutic proteins. Recent …
Virus-like particles in vaccine development
A Roldão, MCM Mellado, LR Castilho… - Expert review of …, 2010 - Taylor & Francis
Virus-like particles (VLPs) are multiprotein structures that mimic the organization and
conformation of authentic native viruses but lack the viral genome, potentially yielding safer …
conformation of authentic native viruses but lack the viral genome, potentially yielding safer …
[HTML][HTML] Apoptosis: A mammalian cell bioprocessing perspective
AL Grilo, A Mantalaris - Biotechnology advances, 2019 - Elsevier
Apoptosis is a form of programmed and controlled cell death that accounts for the majority of
cellular death in bioprocesses. Cell death affects culture longevity and product quality; it is …
cellular death in bioprocesses. Cell death affects culture longevity and product quality; it is …
[HTML][HTML] SiO2 nanoparticles induce cytotoxicity and protein expression alteration in HaCaT cells
X Yang, J Liu, H He, L Zhou, C Gong, X Wang… - Particle and fibre …, 2010 - Springer
Background Nanometer silicon dioxide (nano-SiO 2) has a wide variety of applications in
material sciences, engineering and medicine; however, the potential cell biological and …
material sciences, engineering and medicine; however, the potential cell biological and …
Expansion of human mesenchymal stem/stromal cells (hMSCs) in bioreactors using microcarriers: lessons learnt and what the future holds
PS Couto, MC Rotondi, A Bersenev, CJ Hewitt… - Biotechnology …, 2020 - Elsevier
Human mesenchymal stem/stromal cells (hMSCs) present a key therapeutic cellular
intervention for use in cell and gene therapy (CGT) applications due to their …
intervention for use in cell and gene therapy (CGT) applications due to their …
Expansion of human mesenchymal stem cells on microcarriers
The effects on human mesenchymal stem cell growth of choosing either of two spinner flask
impeller geometries, two microcarrier concentrations and two cell concentrations (seeding …
impeller geometries, two microcarrier concentrations and two cell concentrations (seeding …
Two-dimensional gel electrophoresis and 2D-DIGE
P Meleady - Difference gel electrophoresis: methods and protocols, 2018 - Springer
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) continues to be one of the
most versatile and widely used techniques to study the proteome of a biological system. In …
most versatile and widely used techniques to study the proteome of a biological system. In …
A cellular response linking eIF4AI activity to eIF4AII transcription
The recruitment of ribosomes to eukaryotic cellular mRNAs requires the activity of two
prototypic RNA helicases, eukaryotic initiation factor (eIF) 4AI and eIF4AII. The eIF4A …
prototypic RNA helicases, eukaryotic initiation factor (eIF) 4AI and eIF4AII. The eIF4A …
[HTML][HTML] pEVL: a linear plasmid for generating mRNA IVT templates with extended encoded poly (A) sequences
AE Grier, S Burleigh, J Sahni, CA Clough… - … Therapy-Nucleic Acids, 2016 - cell.com
Increasing demand for large-scale synthesis of in vitro transcribed (IVT) mRNA is being
driven by the increasing use of mRNA for transient gene expression in cell engineering and …
driven by the increasing use of mRNA for transient gene expression in cell engineering and …
A review on the current methods of Chinese hamster ovary (CHO) cells cultivation for the production of therapeutic protein
SM Sharker, A Rahman - Current Drug Discovery Technologies, 2021 - ingentaconnect.com
Most of the clinical approved protein-based drugs or under clinical trials have a profound
impact on the treatment of critical diseases. The mammalian eukaryotic cells culture …
impact on the treatment of critical diseases. The mammalian eukaryotic cells culture …