Chromosomes occupy distinct territories in the interphase cell nucleus. These chromosome
territories are non-randomly arranged within the nuclear space. We are only just uncovering …

A cell fraction that would today be termed “the nuclear matrix” was first described and
patented in 1948 by Russian investigators. In 1974 this fraction was rediscovered and …

Spatiotemporal patterns of gene expression are fundamental to every developmental
program. The resulting macroscopic domains have been mainly characterized by their levels …

Early embryonic patterning events are strikingly precise, a fact that appears incompatible
with the stochastic gene expression observed across phyla. Using single-molecule mRNA …

Quiescent stem cells in adult tissues can be activated for homeostasis or repair. Neural stem
cells (NSCs) in Drosophila are reactivated from quiescence in response to nutrition by the …

Maternally inherited mitochondria and other cytoplasmic organelles play essential roles
supporting the development of early embryos and their germ cells. Using methods that …

The expression of Sonic hedgehog (Shh) in mouse limb buds is regulated by a long-range
enhancer 1 Mb upstream of the Shh promoter. We used 3D-FISH and chromosome …

Abstract oskar messenger RNA localization at the posterior pole of the Drosophila oocyte is
essential for germline and abdomen formation in the future embryo,. The nuclear shuttling …

We present a fluorescence-based, multiplex in situ hybridization method that permits the
simultaneous detection of five differently labeled antisense RNA probes and up to seven …

In Drosophila oocytes, precise localization of the posterior determinant, Oskar, is required for
posterior patterning. This precision is accomplished by a localization-dependent …