Chromosome positioning in the interphase nucleus

LA Parada, T Misteli - Trends in cell biology, 2002 - cell.com
Chromosomes occupy distinct territories in the interphase cell nucleus. These chromosome
territories are non-randomly arranged within the nuclear space. We are only just uncovering …

Half a century of “the nuclear matrix”

T Pederson - Molecular biology of the cell, 2000 - Am Soc Cell Biol
A cell fraction that would today be termed “the nuclear matrix” was first described and
patented in 1948 by Russian investigators. In 1974 this fraction was rediscovered and …

Quantitative imaging of transcription in living Drosophila embryos links polymerase activity to patterning

HG Garcia, M Tikhonov, A Lin, T Gregor - Current biology, 2013 - cell.com
Spatiotemporal patterns of gene expression are fundamental to every developmental
program. The resulting macroscopic domains have been mainly characterized by their levels …

[HTML][HTML] Precise developmental gene expression arises from globally stochastic transcriptional activity

SC Little, M Tikhonov, T Gregor - Cell, 2013 - cell.com
Early embryonic patterning events are strikingly precise, a fact that appears incompatible
with the stochastic gene expression observed across phyla. Using single-molecule mRNA …

Cell cycle heterogeneity directs the timing of neural stem cell activation from quiescence

L Otsuki, AH Brand - Science, 2018 - science.org
Quiescent stem cells in adult tissues can be activated for homeostasis or repair. Neural stem
cells (NSCs) in Drosophila are reactivated from quiescence in response to nutrition by the …

A Balbiani body and the fusome mediate mitochondrial inheritance duringDrosophila oogenesis

RT Cox, AC Spradling - 2003 - journals.biologists.com
Maternally inherited mitochondria and other cytoplasmic organelles play essential roles
supporting the development of early embryos and their germ cells. Using methods that …

[HTML][HTML] Chromosomal dynamics at the Shh locus: limb bud-specific differential regulation of competence and active transcription

T Amano, T Sagai, H Tanabe, Y Mizushina… - Developmental cell, 2009 - cell.com
The expression of Sonic hedgehog (Shh) in mouse limb buds is regulated by a long-range
enhancer 1 Mb upstream of the Shh promoter. We used 3D-FISH and chromosome …

Splicing of oskar RNA in the nucleus is coupled to its cytoplasmic localization

O Hachet, A Ephrussi - Nature, 2004 - nature.com
Abstract oskar messenger RNA localization at the posterior pole of the Drosophila oocyte is
essential for germline and abdomen formation in the future embryo,. The nuclear shuttling …

Multiplex Detection of RNA Expression in Drosophila Embryos

D Kosman, CM Mizutani, D Lemons, WG Cox… - Science, 2004 - science.org
We present a fluorescence-based, multiplex in situ hybridization method that permits the
simultaneous detection of five differently labeled antisense RNA probes and up to seven …

Cup is an eIF4E binding protein required for both the translational repression of oskar and the recruitment of Barentsz

JE Wilhelm, M Hilton, Q Amos, WJ Henzel - The Journal of cell biology, 2003 - rupress.org
In Drosophila oocytes, precise localization of the posterior determinant, Oskar, is required for
posterior patterning. This precision is accomplished by a localization-dependent …