Structural biology of CRISPR–Cas immunity and genome editing enzymes
CRISPR–Cas systems provide resistance against foreign mobile genetic elements and have
a wide range of genome editing and biotechnological applications. In this Review, we …
a wide range of genome editing and biotechnological applications. In this Review, we …
Discovery of diverse CRISPR-Cas systems and expansion of the genome engineering toolbox
CRISPR systems mediate adaptive immunity in bacteria and archaea through diverse
effector mechanisms and have been repurposed for versatile applications in therapeutics …
effector mechanisms and have been repurposed for versatile applications in therapeutics …
Structural basis for mismatch surveillance by CRISPR–Cas9
CRISPR–Cas9 as a programmable genome editing tool is hindered by off-target DNA
cleavage,,–, and the underlying mechanisms by which Cas9 recognizes mismatches are …
cleavage,,–, and the underlying mechanisms by which Cas9 recognizes mismatches are …
Diverse virus-encoded CRISPR-Cas systems include streamlined genome editors
CRISPR-Cas systems are host-encoded pathways that protect microbes from viral infection
using an adaptive RNA-guided mechanism. Using genome-resolved metagenomics, we find …
using an adaptive RNA-guided mechanism. Using genome-resolved metagenomics, we find …
TnpB structure reveals minimal functional core of Cas12 nuclease family
G Sasnauskas, G Tamulaitiene, G Druteika, A Carabias… - Nature, 2023 - nature.com
The widespread TnpB proteins of IS200/IS605 transposon family have recently emerged as
the smallest RNA-guided nucleases capable of targeted genome editing in eukaryotic cells …
the smallest RNA-guided nucleases capable of targeted genome editing in eukaryotic cells …
Cas12n nucleases, early evolutionary intermediates of type V CRISPR, comprise a distinct family of miniature genome editors
W Chen, J Ma, Z Wu, Z Wang, H Zhang, W Fu, D Pan… - Molecular cell, 2023 - cell.com
Type V CRISPR-associated systems (Cas) 12 family nucleases are considered to have
evolved from transposon-associated TnpB, and several of these nucleases have been …
evolved from transposon-associated TnpB, and several of these nucleases have been …
Targeting miRNA by CRISPR/Cas in cancer: advantages and challenges
Clustered regulatory interspaced short palindromic repeats (CRISPR) has changed
biomedical research and provided entirely new models to analyze every aspect of …
biomedical research and provided entirely new models to analyze every aspect of …
Structure and engineering of miniature Acidibacillus sulfuroxidans Cas12f1
Z Wu, D Liu, D Pan, H Yu, J Shi, J Ma, W Fu, Z Wang… - Nature Catalysis, 2023 - nature.com
The miniature CRISPR-Cas12f nucleases enable efficient delivery via cargo-size-limited
vehicles, thereby showing promise for in vivo therapeutic applications. Acidibacillus …
vehicles, thereby showing promise for in vivo therapeutic applications. Acidibacillus …
Genome editing in plants using the compact editor CasΦ
Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins
(CRISPR-Cas) systems have been developed as important tools for plant genome …
(CRISPR-Cas) systems have been developed as important tools for plant genome …
RNA targeting unleashes indiscriminate nuclease activity of CRISPR–Cas12a2
Cas12a2 is a CRISPR-associated nuclease that performs RNA-guided, sequence-
nonspecific degradation of single-stranded RNA, single-stranded DNA and double-stranded …
nonspecific degradation of single-stranded RNA, single-stranded DNA and double-stranded …