Applications of PCR-based tools for detection and identification of animal trypanosomes: a review and perspectives

M Desquesnes, AMR Dávila - Veterinary parasitology, 2002 - Elsevier
This paper aims to review the applications of the polymerase chain reaction (PCR) for the
detection and identification of trypanosomes in animals. The diagnosis of trypanosomes …

Options available—from start to finish—for obtaining data from DNA microarrays II

AJ Holloway, RK Van Laar, RW Tothill, DDL Bowtell - Nature genetics, 2002 - nature.com
Microarray technology has undergone a rapid evolution. With widespread interest in large-
scale genomic research, an abundance of equipment and reagents have now become …

TM4: a free, open-source system for microarray data management and analysis

AI Saeed, V Sharov, J White, J Li, W Liang… - …, 2003 - Taylor & Francis
BioTechniques 34: 374-378 (February 2003) supported, MADAM is being adapted to read
and write MAGE-ML, the XML data exchange format being developed by an international …

Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites

TN Siegel, DR Hekstra, X Wang, S Dewell… - Nucleic acids …, 2010 - academic.oup.com
Transcription of protein-coding genes in trypanosomes is polycistronic and gene expression
is primarily regulated by post-transcriptional mechanisms. Sequence motifs in the …

Genomic organization and expression profile of the mucin-associated surface protein (masp) family of the human pathogen Trypanosoma cruzi

DC Bartholomeu, GC Cerqueira, ACA Leao… - Nucleic acids …, 2009 - academic.oup.com
A novel large multigene family was recently identified in the human pathogen Trypanosoma
cruzi, causative agent of Chagas disease, and corresponds to∼ 6% of the parasite diploid …

The small chromosomes of Trypanosoma brucei involved in antigenic variation are constructed around repetitive palindromes

B Wickstead, K Ersfeld, K Gull - Genome research, 2004 - genome.cshlp.org
Most eukaryotic genomes contain large regions of satellite DNA. These arrays are often
associated with essential chromosomal functions, but remain largely absent from genome …

Host specificity and incidence of Trypanosoma in some African rainforest birds: a molecular approach

RNM Sehgal, HI Jones, TB Smith - Molecular Ecology, 2001 - Wiley Online Library
Studies of host–parasite interactions in birds have contributed greatly to our understanding
of the evolution and ecology of disease. Here we employ molecular techniques to determine …

Coordination of cell cycle and cytokinesis in Trypanosoma brucei

PG McKean - Current opinion in microbiology, 2003 - Elsevier
In common with all eukaryotic cells, trypanosomes must coordinate a complex series of
morphogenetic events both temporally and spatially during the cell cycle. The structural and …

Evolutionary cell biology of chromosome segregation: insights from trypanosomes

B Akiyoshi, K Gull - Open biology, 2013 - royalsocietypublishing.org
Faithful transmission of genetic material is essential for the survival of all organisms.
Eukaryotic chromosome segregation is driven by the kinetochore that assembles onto …

[HTML][HTML] RNA interference of a trypanosome topoisomerase II causes progressive loss of mitochondrial DNA

Z Wang, PT Englund - The EMBO journal, 2001 - embopress.org
We studied the function of a Trypanosoma brucei topoisomerase II using RNA interference
(RNAi). Expression of a topoisomerase II double‐stranded RNA as a stem–loop caused …