[HTML][HTML] Animal board invited review: an update on the methods for semen quality evaluation in swine–from farm to the lab
C Maside, S Recuero, A Salas-Huetos… - animal, 2023 - Elsevier
Pig breeding is mainly conducted through artificial insemination with liquid-stored semen. It
is, therefore, crucial to ensure that sperm quality is over the standard thresholds, as reduced …
is, therefore, crucial to ensure that sperm quality is over the standard thresholds, as reduced …
Methodological considerations for examining the relationship between sperm morphology and motility
Sperm cells of all taxa share a common goal to reach and fertilize an ovum, yet sperm are
one of the most diverse cell types in nature. While the structural diversity of these cells is well …
one of the most diverse cell types in nature. While the structural diversity of these cells is well …
[HTML][HTML] Predicting fertility from sperm motility landscapes
P Fernández-López, J Garriga, I Casas… - Communications …, 2022 - nature.com
Understanding the organisational principles of sperm motility has both evolutionary and
applied impact. The emergence of computer aided systems in this field came with the …
applied impact. The emergence of computer aided systems in this field came with the …
[HTML][HTML] Protoplast Isolation and Shoot Regeneration from Protoplast-Derived Callus of Petunia hybrida Cv. Mirage Rose
Despite the increasing use of protoplasts in plant biotechnology research, shoot
regeneration from protoplasts remains challenging. In this study, we investigated the factors …
regeneration from protoplasts remains challenging. In this study, we investigated the factors …
[HTML][HTML] ProAKAP4 as novel molecular marker of sperm quality in ram: an integrative study in fresh, cooled and cryopreserved sperm
M F. Riesco, L Anel-Lopez, M Neila-Montero… - Biomolecules, 2020 - mdpi.com
To improve artificial insemination protocols in ovine species it is crucial to optimize sperm
quality evaluation after preservation technologies. Emerging technologies based on novel …
quality evaluation after preservation technologies. Emerging technologies based on novel …
Validation of a new multiparametric protocol to assess viability, acrosome integrity and mitochondrial activity in cooled and frozen thawed boar spermatozoa
RA Gonzalez‐Castro, FJ Peña… - Cytometry Part B …, 2022 - Wiley Online Library
Background Motility, morphology, membrane integrity and DNA fragmentation are sperm
characteristics routinely used to assess quality of boar spermatozoa. However, the …
characteristics routinely used to assess quality of boar spermatozoa. However, the …
Assessment of sperm mitochondrial activity by flow cytometry and fluorescent microscopy: a comparative study of mitochondrial fluorescent probes in bovine …
M Llavanera, B Mislei, O Blanco-Prieto… - Reproduction …, 2022 - CSIRO Publishing
Context While conventional semen analysis is a simple, time-saving, and economical means
to evaluate sperm quality, it leaves biochemical and metabolic characteristics of …
to evaluate sperm quality, it leaves biochemical and metabolic characteristics of …
Assessments of sperm quality integrating morphology, swimming patterns, bioenergetics and cell signalling
ERS Roldan - Theriogenology, 2020 - Elsevier
Spermatozoa are diverse in form and function and these differences impact on their
fertilizing capacity. Because of considerable inter-male and inter-species differences in …
fertilizing capacity. Because of considerable inter-male and inter-species differences in …
A 5-color flow cytometry panel to assess plasma membrane integrity, acrosomal status, membrane lipid organization and mitochondrial activity of boar and stallion …
M Quirino, U Jakop, APG Mellagi, FP Bortolozzo… - Animal Reproduction …, 2022 - Elsevier
For a more practically applicable analysis of different sperm characteristics, this study aimed
to develop a 5-color flow cytometry (FC) panel to concurrently analyze four sperm …
to develop a 5-color flow cytometry (FC) panel to concurrently analyze four sperm …
[HTML][HTML] Complete chromatin decondensation of pig sperm is required to analyze sperm DNA breaks with the comet assay
J Ribas-Maynou, A Delgado-Bermúdez… - Frontiers in Cell and …, 2021 - frontiersin.org
Sperm quality is usually evaluated prior to artificial insemination in farm animals. In addition
to conventional semen analysis, other biomarkers, such as mitochondrial activity, integrity …
to conventional semen analysis, other biomarkers, such as mitochondrial activity, integrity …