[HTML][HTML] Animal board invited review: an update on the methods for semen quality evaluation in swine–from farm to the lab

C Maside, S Recuero, A Salas-Huetos… - animal, 2023 - Elsevier
Pig breeding is mainly conducted through artificial insemination with liquid-stored semen. It
is, therefore, crucial to ensure that sperm quality is over the standard thresholds, as reduced …

Methodological considerations for examining the relationship between sperm morphology and motility

KA Hook, HS Fisher - Molecular reproduction and development, 2020 - Wiley Online Library
Sperm cells of all taxa share a common goal to reach and fertilize an ovum, yet sperm are
one of the most diverse cell types in nature. While the structural diversity of these cells is well …

[HTML][HTML] Predicting fertility from sperm motility landscapes

P Fernández-López, J Garriga, I Casas… - Communications …, 2022 - nature.com
Understanding the organisational principles of sperm motility has both evolutionary and
applied impact. The emergence of computer aided systems in this field came with the …

[HTML][HTML] Protoplast Isolation and Shoot Regeneration from Protoplast-Derived Callus of Petunia hybrida Cv. Mirage Rose

HH Kang, AH Naing, CK Kim - Biology, 2020 - mdpi.com
Despite the increasing use of protoplasts in plant biotechnology research, shoot
regeneration from protoplasts remains challenging. In this study, we investigated the factors …

[HTML][HTML] ProAKAP4 as novel molecular marker of sperm quality in ram: an integrative study in fresh, cooled and cryopreserved sperm

M F. Riesco, L Anel-Lopez, M Neila-Montero… - Biomolecules, 2020 - mdpi.com
To improve artificial insemination protocols in ovine species it is crucial to optimize sperm
quality evaluation after preservation technologies. Emerging technologies based on novel …

Validation of a new multiparametric protocol to assess viability, acrosome integrity and mitochondrial activity in cooled and frozen thawed boar spermatozoa

RA Gonzalez‐Castro, FJ Peña… - Cytometry Part B …, 2022 - Wiley Online Library
Background Motility, morphology, membrane integrity and DNA fragmentation are sperm
characteristics routinely used to assess quality of boar spermatozoa. However, the …

Assessment of sperm mitochondrial activity by flow cytometry and fluorescent microscopy: a comparative study of mitochondrial fluorescent probes in bovine …

M Llavanera, B Mislei, O Blanco-Prieto… - Reproduction …, 2022 - CSIRO Publishing
Context While conventional semen analysis is a simple, time-saving, and economical means
to evaluate sperm quality, it leaves biochemical and metabolic characteristics of …

Assessments of sperm quality integrating morphology, swimming patterns, bioenergetics and cell signalling

ERS Roldan - Theriogenology, 2020 - Elsevier
Spermatozoa are diverse in form and function and these differences impact on their
fertilizing capacity. Because of considerable inter-male and inter-species differences in …

A 5-color flow cytometry panel to assess plasma membrane integrity, acrosomal status, membrane lipid organization and mitochondrial activity of boar and stallion …

M Quirino, U Jakop, APG Mellagi, FP Bortolozzo… - Animal Reproduction …, 2022 - Elsevier
For a more practically applicable analysis of different sperm characteristics, this study aimed
to develop a 5-color flow cytometry (FC) panel to concurrently analyze four sperm …

[HTML][HTML] Complete chromatin decondensation of pig sperm is required to analyze sperm DNA breaks with the comet assay

J Ribas-Maynou, A Delgado-Bermúdez… - Frontiers in Cell and …, 2021 - frontiersin.org
Sperm quality is usually evaluated prior to artificial insemination in farm animals. In addition
to conventional semen analysis, other biomarkers, such as mitochondrial activity, integrity …