Genetic engineering applications in the field of biofuel are rapidly expanding due to their
potential to boost biomass productivity while lowering its cost and enhancing its quality …

Synthetic biology, with its goal of designing biological entities for wide-ranging purposes,
remains a field of intensive research interest. However, the vast complexity of biological …

Engineering cellular metabolism for improved production of valuable chemicals requires
extensive modulation of bacterial genome to explore complex genetic spaces. Here, we …

Carboxylic acids are an attractive biorenewable chemical. However, like many other
fermentatively produced compounds, they are inhibitory to the biocatalyst. An understanding …

Today, the dramatic changes in types of food consumed have led to an increased burden of
chronic diseases. Therefore, the emphasis of food research is not only to ensure quality food …

A fundamental challenge in basic and applied biology is to reprogram cells with improved or
novel traits on a genomic scale. However, the current ability to reprogram a cell on the …

Background Low ethanol tolerance of Kluyveromyces marxianus limits its application in high-
temperature ethanol fermentation. As a complex phenotype, ethanol tolerance involves …

Background High temperature inhibits cell growth and ethanol fermentation of
Saccharomyces cerevisiae. As a complex phenotype, thermotolerance usually involves …

Cumulative site‐directed mutagenesis is of limited suitability for the global analysis of the
gene functions in the microbe's cellular network. In order to simplify and stabilize the …

Genome engineering strategies—such as genome editing, reduction and shuffling, and de
novo genome synthesis—enable the modification of specific genomic locations in a directed …