Cell-free gene expression: an expanded repertoire of applications
Cell-free biology is the activation of biological processes without the use of intact living cells.
It has been used for more than 50 years across the life sciences as a foundational research …
It has been used for more than 50 years across the life sciences as a foundational research …
Hydrogels as functional components in artificial cell systems
Recent years have seen substantial efforts aimed at constructing artificial cells from various
molecular components with the aim of mimicking the processes, behaviours and …
molecular components with the aim of mimicking the processes, behaviours and …
Compartmentalizing cell-free systems: toward creating life-like artificial cells and beyond
Building an artificial cell is a research area that is rigorously studied in the field of synthetic
biology. It has brought about much attention with the aim of ultimately constructing a natural …
biology. It has brought about much attention with the aim of ultimately constructing a natural …
Bottom-up construction of complex biomolecular systems with cell-free synthetic biology
N Laohakunakorn, L Grasemann… - … in bioengineering and …, 2020 - frontiersin.org
Cell-free systems offer a promising approach to engineer biology since their open nature
allows for well-controlled and characterized reaction conditions. In this review, we discuss …
allows for well-controlled and characterized reaction conditions. In this review, we discuss …
Hydrogels for 3D mammalian cell culture: a starting guide for laboratory practice
F Ruedinger, A Lavrentieva, C Blume… - Applied microbiology …, 2015 - Springer
Hydrogels have become one of the most popular platforms for three-dimensional (3D)
cultivation of mammalian cells. The enormous versatility of hydrogel materials makes it …
cultivation of mammalian cells. The enormous versatility of hydrogel materials makes it …
Biocompatible fluorinated polyglycerols for droplet microfluidics as an alternative to PEG-based copolymer surfactants
In droplet-based microfluidics, non-ionic, high-molecular weight surfactants are required to
stabilize droplet interfaces. One of the most common structures that imparts stability as well …
stabilize droplet interfaces. One of the most common structures that imparts stability as well …
Lab on a particle technologies
From “Lab on a Chip” to “Lab on a Particle”. The miniaturization and automation of life
science laboratory operations has leveraged fabrication advances in the integrated circuit …
science laboratory operations has leveraged fabrication advances in the integrated circuit …
Artificial gel‐based organelles for spatial organization of cell‐free gene expression reactions
L Aufinger, FC Simmel - Angewandte Chemie International …, 2018 - Wiley Online Library
Gel‐based artificial organelles have been developed that enable sequence‐specific and
programmable localization of cell‐free transcription and translation reactions inside an …
programmable localization of cell‐free transcription and translation reactions inside an …
Lipid sponge droplets as programmable synthetic organelles
A Bhattacharya, H Niederholtmeyer… - Proceedings of the …, 2020 - National Acad Sciences
Living cells segregate molecules and reactions in various subcellular compartments known
as organelles. Spatial organization is likely essential for expanding the biochemical …
as organelles. Spatial organization is likely essential for expanding the biochemical …