Over the past few decades, Escherichia coli (E. coli) remains the most favorable host among
the microbial cell factories for the production of soluble recombinant proteins. Recombinant …

The production of high yields of soluble recombinant protein is one of the main objectives of
protein biotechnology. Several factors, such as expression system, vector, host, media …

The endoxylanase XT6 produced by Geobacillus stearothermophilus is a desirable
candidate for industrial applications. In this study, the gene encoding XT6 was cloned using …

Response surface methodology (RSM) and artificial neural network (ANN) were used to
optimize the effect of four independent variables, viz. glucose, sodium chloride (NaCl) …

Caffeine, a significant naturally occurring alkaloid in beverages like tea and coffee, can be
degraded by bacteria. Prolonged caffeine consumption can stimulate adrenal glands, cause …

Phenol is a toxic compound and is one of the major pollutants contained in the waste water
from petroleum and its downstream industries. Response surface methodology (RSM) was …

Liangzhou fumigated vinegar is famous in China for its distinct flavor. How to enhance flavor
in traditional fermented foods, such as vinegar, has attracted a large interest in recent years …

Biomass conversion to an utilizable energy sources such as monomer sugars using
enzymatic hydrolysis has been emerged as the current technology which promises the …

Escherichia coli BL21 (DE3) with a plasmid vector pET-22b (+) carrying xylanase coding
gene isolated from an extremely thermophilic bacterium, Thermotoga neapolitana was used …

Several medium formulations were screened for the production of a thermostable and
organic solvent tolerant lipase by a recombinant Escherichia coli BL21. The highest lipase …