[HTML][HTML] Past, present, and future perspectives of environmental DNA (eDNA) metabarcoding: A systematic review in methods, monitoring, and applications of global …

KM Ruppert, RJ Kline, MS Rahman - Global Ecology and Conservation, 2019 - Elsevier
Environmental DNA (eDNA) metabarcoding is a novel method of assessing biodiversity
wherein samples are taken from the environment via water, sediment or air from which DNA …

[HTML][HTML] DNA barcode reference libraries for the monitoring of aquatic biota in Europe: Gap-analysis and recommendations for future work

H Weigand, AJ Beermann, F Čiampor, FO Costa… - Science of the Total …, 2019 - Elsevier
Effective identification of species using short DNA fragments (DNA barcoding and DNA
metabarcoding) requires reliable sequence reference libraries of known taxa. Both …

Meta‐analysis shows both congruence and complementarity of DNA and eDNA metabarcoding to traditional methods for biological community assessment

F Keck, RC Blackman, R Bossart… - Molecular …, 2022 - Wiley Online Library
DNA metabarcoding is increasingly used for the assessment of aquatic communities, and
numerous studies have investigated the consistency of this technique with traditional …

Diat. barcode, an open-access curated barcode library for diatoms

F Rimet, E Gusev, M Kahlert, MG Kelly… - Scientific Reports, 2019 - nature.com
Diatoms (Bacillariophyta) are ubiquitous microalgae which produce a siliceous exoskeleton
and which make a major contribution to the productivity of oceans and freshwaters. They …

Genetic markers for metabarcoding of freshwater microalgae

E Kezlya, N Tseplik, M Kulikovskiy - Biology, 2023 - mdpi.com
Simple Summary The metabarcoding approach is widely used for studying the diversity and
distribution of freshwater microalgae and for routine biomonitoring. Due to microalgae being …

Evaluation and sensitivity analysis of diatom DNA metabarcoding for WFD bioassessment of Mediterranean rivers

J Pérez-Burillo, R Trobajo, V Vasselon, F Rimet… - Science of The Total …, 2020 - Elsevier
Our study of 164 diatom samples from Catalonia (NE Spain) is the first to evaluate the
applicability of DNA metabarcoding, based on high throughput sequencing (HTS) using a …

DNA metabarcoding reveals differences in distribution patterns and ecological preferences among genetic variants within some key freshwater diatom species

J Pérez-Burillo, R Trobajo, M Leira, F Keck… - Science of the Total …, 2021 - Elsevier
Our study evaluates differences in the distribution and ecology of genetic variants within
several ecologically important diatom species that are also key for Water Framework …

Benthic diatoms in river biomonitoring—present and future perspectives within the water framework directive

A Masouras, I Karaouzas, E Dimitriou, G Tsirtsis… - Water, 2021 - mdpi.com
The European Water Framework Directive 2000/60/EC (WFD) has been implemented over
the past 20 years, using physicochemical, biological and hydromorphological elements to …

Diatom metabarcoding applied to large scale monitoring networks: Optimization of bioinformatics strategies using Mothur software

SF Rivera, V Vasselon, A Bouchez, F Rimet - Ecological indicators, 2020 - Elsevier
Benthic diatoms are routinely used as ecological indicators in rivers. A standardized
methodology is based on biofilm sampling, species identification, and counting under …

[HTML][HTML] The potential of exact sequence variants (ESVs) to interpret and assess the impact of agricultural pressure on stream diatom assemblages revealed by DNA …

K Tapolczai, GB Selmeczy, B Szabó, B Viktória… - Ecological …, 2021 - Elsevier
Land-use imposes an important potential threat on the aquatic ecosystems of riverine
habitats. In this study, DNA metabarcoding was used to assess the effect of land-use on …