The arms race between bacteria and their phage foes

HG Hampton, BNJ Watson, PC Fineran - Nature, 2020 - nature.com
Bacteria are under immense evolutionary pressure from their viral invaders—
bacteriophages. Bacteria have evolved numerous immune mechanisms, both innate and …

The CRISPR-Cas mechanism for adaptive immunity and alternate bacterial functions fuels diverse biotechnologies

S Newsom, HP Parameshwaran, L Martin… - Frontiers in cellular and …, 2021 - frontiersin.org
Bacterial and archaeal CRISPR-Cas systems offer adaptive immune protection against
foreign mobile genetic elements (MGEs). This function is regulated by sequence specific …

Uncovering the functional diversity of rare CRISPR-Cas systems with deep terascale clustering

H Altae-Tran, S Kannan, AJ Suberski, KS Mears… - Science, 2023 - science.org
Microbial systems underpin many biotechnologies, including CRISPR, but the exponential
growth of sequence databases makes it difficult to find previously unidentified systems. In …

Type IV CRISPR–Cas systems are highly diverse and involved in competition between plasmids

R Pinilla-Redondo, D Mayo-Muñoz… - Nucleic acids …, 2020 - academic.oup.com
CRISPR–Cas systems provide prokaryotes with adaptive immune functions against viruses
and other genetic parasites. In contrast to all other types of CRISPR–Cas systems, type IV …

Chemistry of Class 1 CRISPR-Cas effectors: Binding, editing, and regulation

TY Liu, JA Doudna - Journal of Biological Chemistry, 2020 - ASBMB
Among the multiple antiviral defense mechanisms found in prokaryotes, CRISPR-Cas
systems stand out as the only known RNA-programmed pathways for detecting and …

Endogenous type I CRISPR-Cas: from foreign DNA defense to prokaryotic engineering

Y Zheng, J Li, B Wang, J Han, Y Hao… - … in bioengineering and …, 2020 - frontiersin.org
Establishment of production platforms through prokaryotic engineering in microbial
organisms would be one of the most efficient means for chemicals, protein, and biofuels …

Type IV-A CRISPR-Csf complex: Assembly, dsDNA targeting, and CasDinG recruitment

N Cui, JT Zhang, Y Liu, Y Liu, XY Liu, C Wang… - Molecular Cell, 2023 - cell.com
Summary Type IV CRISPR-Cas systems, which are primarily found on plasmids and exhibit
a strong plasmid-targeting preference, are the only one of the six known CRISPR-Cas types …

A Type IV-A CRISPR-Cas System in Pseudomonas aeruginosa Mediates RNA-Guided Plasmid Interference In Vivo

VM Crowley, A Catching, HN Taylor, AL Borges… - The CRISPR …, 2019 - liebertpub.com
Bacteria and archaea use CRISPR-Cas adaptive immune systems to destroy
complementary nucleic acids using RNAs derived from CRISPR loci. Here, we provide the …

Evolution of Type IV CRISPR-Cas Systems: Insights from CRISPR Loci in Integrative Conjugative Elements of Acidithiobacillia

A Moya-Beltrán, KS Makarova, LG Acuña… - The CRISPR …, 2021 - liebertpub.com
Type IV CRISPR-Cas are a distinct variety of highly derived CRISPR-Cas systems that
appear to have evolved from type III systems through the loss of the target-cleaving nuclease …

Positioning diverse type IV structures and functions within class 1 CRISPR-Cas systems

HN Taylor, E Laderman, M Armbrust… - Frontiers in …, 2021 - frontiersin.org
Type IV CRISPR systems encode CRISPR associated (Cas)-like proteins that combine with
small RNAs to form multi-subunit ribonucleoprotein complexes. However, the lack of Cas …