[HTML][HTML] Therapeutic glycoprotein production in mammalian cells

ME Lalonde, Y Durocher - Journal of biotechnology, 2017 - Elsevier
Over the last years, the biopharmaceutical industry has significantly turned its biologics
production towards mammalian cell expression systems. The presence of glycosylation …

The emerging CHO systems biology era: harnessing the 'omics revolution for biotechnology

HF Kildegaard, D Baycin-Hizal, NE Lewis… - Current opinion in …, 2013 - Elsevier
Chinese hamster ovary (CHO) cells are the primary factories for biopharmaceuticals
because of their capacity to correctly fold and post-translationally modify recombinant …

De novo assembly and characterization of root transcriptome using Illumina paired-end sequencing and development of cSSR markers in sweetpotato (Ipomoea …

Z Wang, B Fang, J Chen, X Zhang, Z Luo, L Huang… - BMC genomics, 2010 - Springer
Background The tuberous root of sweetpotato is an important agricultural and biological
organ. There are not sufficient transcriptomic and genomic data in public databases for …

De novo assembly and characterization of bark transcriptome using Illumina sequencing and development of EST-SSR markers in rubber tree (Hevea brasiliensis …

D Li, Z Deng, B Qin, X Liu, Z Men - BMC genomics, 2012 - Springer
Background In rubber tree, bark is one of important agricultural and biological organs.
However, the molecular mechanism involved in the bark formation and development in …

Comprehensive analysis of the Corynebacterium glutamicum transcriptome using an improved RNAseq technique

K Pfeifer-Sancar, A Mentz, C Rückert, J Kalinowski - BMC genomics, 2013 - Springer
Abstract Background The use of RNAseq to resolve the transcriptional organization of an
organism was established in recent years and also showed the complexity and dynamics of …

Directional RNA deep sequencing sheds new light on the transcriptional response of Anabaena sp. strain PCC 7120 to combined-nitrogen deprivation

BL Flaherty, F Van Nieuwerburgh, SR Head… - BMC genomics, 2011 - Springer
Background Cyanobacteria are potential sources of renewable chemicals and biofuels and
serve as model organisms for bacterial photosynthesis, nitrogen fixation, and responses to …

Genomic sequencing and analysis of a Chinese hamster ovary cell line using Illumina sequencing technology

S Hammond, JC Swanberg, M Kaplarevic, KH Lee - BMC genomics, 2011 - Springer
Background Chinese hamster ovary (CHO) cells are among the most widely used hosts for
therapeutic protein production. Yet few genomic resources are available to aid in …

Unraveling the Chinese hamster ovary cell line transcriptome by next-generation sequencing

J Becker, M Hackl, O Rupp, T Jakobi, J Schneider… - Journal of …, 2011 - Elsevier
The pyrosequencing technology from 454 Life Sciences and a novel assembly approach for
cDNA sequences with the Newbler Assembler were used to achieve a major step forward to …

Construction of a public CHO cell line transcript database using versatile bioinformatics analysis pipelines

O Rupp, J Becker, K Brinkrolf, C Timmermann, N Borth… - PloS one, 2014 - journals.plos.org
Chinese hamster ovary (CHO) cell lines represent the most commonly used mammalian
expression system for the production of therapeutic proteins. In this context, detailed …

[HTML][HTML] Advances in improving mammalian cells metabolism for recombinant protein production

C Altamirano, J Berrios, M Vergara… - Electronic Journal of …, 2013 - SciELO Chile
Background: The production of recombinant proteins for therapeutic use represents a great
impact on the biotechnology industry. In this context, established mammalian cell lines …