Scalable sequence–function studies have enabled the systematic analysis and cataloging of
hundreds of thousands of coding and noncoding genetic variants in the human genome …

After fertilization, the quiescent zygote experiences a burst of genome activation that initiates
a short-lived totipotent state. Understanding the process of totipotency in human cells would …

Increased control of biological growth and form is an essential gateway to transformative
medical advances. Repairing of birth defects, restoring lost or damaged organs, normalizing …

Hematopoietic stem cells (HSCs) are the rare cells responsible for the lifelong curative
effects of hematopoietic cell (HC) transplantation. The demand for clinical-grade HSCs has …

Here, we report inducible mosaic animal for perturbation (iMAP), a transgenic platform
enabling in situ CRISPR targeting of at least 100 genes in parallel throughout the mouse …

Detailed studies of the embryo allow an increasingly mechanistic understanding of
development, which has proved of profound relevance to human disease. The last decade …

Inherited blood disorders comprise a large spectrum of diseases due to germline mutations
in genes with key function in the hematopoietic system; they include immunodeficiencies …

Stem cells have garnered significant attention in regenerative medicine owing to their
abilities of multi-directional differentiation and self-renewal. Despite these encouraging …

Cancer stem cells (CSCs) play an important role in cancer development. Based on
advancements in CSC research, we propose a monophyletic model of cancer. This model is …

Analysis of cellular components at the single-cell level is important to reveal cellular
heterogeneity. However, current technologies to isolate individual cells are either label …