[HTML][HTML] Environmental DNA metabarcoding for benthic monitoring: A review of sediment sampling and DNA extraction methods

J Pawlowski, K Bruce, K Panksep, FI Aguirre… - Science of the Total …, 2022 - Elsevier
Environmental DNA (eDNA) metabarcoding (parallel sequencing of DNA/RNA for
identification of whole communities within a targeted group) is revolutionizing the field of …

[HTML][HTML] The future of biotic indices in the ecogenomic era: Integrating (e) DNA metabarcoding in biological assessment of aquatic ecosystems

J Pawlowski, M Kelly-Quinn, F Altermatt… - Science of the Total …, 2018 - Elsevier
The bioassessment of aquatic ecosystems is currently based on various biotic indices that
use the occurrence and/or abundance of selected taxonomic groups to define ecological …

Ecosystems monitoring powered by environmental genomics: a review of current strategies with an implementation roadmap

T Cordier, L Alonso‐Sáez… - Molecular …, 2021 - Wiley Online Library
A decade after environmental scientists integrated high‐throughput sequencing
technologies in their toolbox, the genomics‐based monitoring of anthropogenic impacts on …

Population‐level inferences from environmental DNA—Current status and future perspectives

EE Sigsgaard, MR Jensen… - Evolutionary …, 2020 - Wiley Online Library
Environmental DNA (eDNA) extracted from water samples has recently shown potential as a
valuable source of population genetic information for aquatic macroorganisms. This …

Can environmental RNA revolutionize biodiversity science?

ME Cristescu - Trends in Ecology & Evolution, 2019 - cell.com
The use of environmental RNA (eRNA) for species identification remains unexplored due to
the observation that in vitro RNA is much less stable than DNA. However, recent lines of …

A comparison of droplet digital polymerase chain reaction (PCR), quantitative PCR and metabarcoding for species‐specific detection in environmental DNA

SA Wood, X Pochon, O Laroche… - Molecular Ecology …, 2019 - Wiley Online Library
Targeted species‐specific and community‐wide molecular diagnostics tools are being used
with increasing frequency to detect invasive or rare species. Few studies have compared the …

Wanted dead or alive? Using metabarcoding of environmental DNA and RNA to distinguish living assemblages for biosecurity applications

X Pochon, A Zaiko, LM Fletcher, O Laroche, SA Wood - PloS one, 2017 - journals.plos.org
High-throughput sequencing metabarcoding studies in marine biosecurity have largely
focused on targeting environmental DNA (eDNA). DNA can persist extracellularly in the …

Embracing environmental genomics and machine learning for routine biomonitoring

T Cordier, A Lanzén, L Apothéloz-Perret-Gentil… - Trends in …, 2019 - cell.com
Genomics is fast becoming a routine tool in medical diagnostics and cutting-edge
biotechnologies. Yet, its use for environmental biomonitoring is still considered a futuristic …

Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples

G Lear, I Dickie, J Banks, S Boyer, HL Buckley… - New Zealand Journal of …, 2018 - JSTOR
Advances in the sequencing of DNA extracted from media such as soil and water offer huge
opportunities for biodiversity monitoring and assessment, particularly where the collection or …

Environmental RNA as a tool for marine community biodiversity assessments

MS Giroux, JR Reichman, T Langknecht… - Scientific Reports, 2022 - nature.com
Microscopic organisms are often overlooked in traditional diversity assessments due to the
difficulty of identifying them based on morphology. Metabarcoding is a method for rapidly …