Nanoparticle delivery of CRISPR/Cas9 for genome editing

L Duan, K Ouyang, X Xu, L Xu, C Wen, X Zhou… - Frontiers in …, 2021 - frontiersin.org
The emerging clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-
associated system (Cas) gene-editing system represents a promising tool for genome …

CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules

W Feng, AM Newbigging, J Tao, Y Cao, H Peng… - Chemical …, 2021 - pubs.rsc.org
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-
associated (Cas) protein systems have transformed the field of genome editing and …

Consensus guidelines for the use and interpretation of angiogenesis assays

P Nowak-Sliwinska, K Alitalo, E Allen, A Anisimov… - Angiogenesis, 2018 - Springer
The formation of new blood vessels, or angiogenesis, is a complex process that plays
important roles in growth and development, tissue and organ regeneration, as well as …

CRISPR-based technologies for the manipulation of eukaryotic genomes

AC Komor, AH Badran, DR Liu - Cell, 2017 - cell.com
The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of
advances in the life sciences that have grown from the ability to edit genomes within living …

Delivery strategies of the CRISPR-Cas9 gene-editing system for therapeutic applications

C Liu, L Zhang, H Liu, K Cheng - Journal of controlled release, 2017 - Elsevier
The CRISPR-Cas9 genome-editing system is a part of the adaptive immune system in
archaea and bacteria to defend against invasive nucleic acids from phages and plasmids …

The new frontier of genome engineering with CRISPR-Cas9

JA Doudna, E Charpentier - Science, 2014 - science.org
Background Technologies for making and manipulating DNA have enabled advances in
biology ever since the discovery of the DNA double helix. But introducing site-specific …

CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes

P Liang, Y Xu, X Zhang, C Ding, R Huang… - Protein & …, 2015 - academic.oup.com
Genome editing tools such as the clustered regularly interspaced short palindromic repeat
(CRISPR)-associated system (Cas) have been widely used to modify genes in model …

CRISPR-Cas systems for editing, regulating and targeting genomes

JD Sander, JK Joung - Nature biotechnology, 2014 - nature.com
Targeted genome editing using engineered nucleases has rapidly gone from being a niche
technology to a mainstream method used by many biological researchers. This widespread …

Non-viral delivery systems for CRISPR/Cas9-based genome editing: Challenges and opportunities

L Li, S Hu, X Chen - Biomaterials, 2018 - Elsevier
In recent years, CRISPR (clustered regularly interspaced short palindromic repeat)/Cas
(CRISPR-associated) genome editing systems have become one of the most robust …

Genome engineering using the CRISPR-Cas9 system

FA Ran, PD Hsu, J Wright, V Agarwala, DA Scott… - Nature protocols, 2013 - nature.com
Targeted nucleases are powerful tools for mediating genome alteration with high precision.
The RNA-guided Cas9 nuclease from the microbial clustered regularly interspaced short …