Strategy to combat biofilms: a focus on biofilm dispersal enzymes

S Wang, Y Zhao, AP Breslawec, T Liang… - npj Biofilms and …, 2023 - nature.com
Bacterial biofilms, which consist of three-dimensional extracellular polymeric substance
(EPS), not only function as signaling networks, provide nutritional support, and facilitate …

[HTML][HTML] Chemical biology tools to probe bacterial glycans

D Calles-Garcia, DH Dube - Current opinion in chemical biology, 2024 - Elsevier
Bacterial cells are covered by a complex carbohydrate coat of armor that allows bacteria to
thrive in a range of environments. As a testament to the importance of bacterial glycans …

Anionic amino acids support hydrolysis of poly-β-(1, 6)-N-acetylglucosamine exopolysaccharides by the biofilm dispersing glycosidase Dispersin B

AP Breslawec, S Wang, C Li, MB Poulin - Journal of Biological Chemistry, 2021 - ASBMB
The exopolysaccharide poly-β-(1→ 6)-N-acetylglucosamine (PNAG) is a major structural
determinant of bacterial biofilms responsible for persistent and nosocomial infections. The …

Multifunctional fluorescent probes for high-throughput characterization of hexosaminidase enzyme activity

S Wang, AP Breslawec, MB Poulin - Bioorganic Chemistry, 2022 - Elsevier
Microbial polysaccharides composed of N-acetylglucosamine (GlcNAc), such as chitin,
peptidoglycan and poly-β-(1→ 6)-GlcNAc (dPNAG), play a critical role in maintaining cell …

[HTML][HTML] Mapping protein–exopolysaccharide binding interaction in Staphylococcus epidermidis biofilms by live cell proximity labeling

LH Vo, S Hong, KE Stepler, SM Liyanaarachchi, J Yang… - bioRxiv, 2023 - ncbi.nlm.nih.gov
Bacterial biofilms consist of cells encased in an extracellular polymeric substance (EPS)
composed of exopolysaccharides, extracellular DNA, and proteins that are critical for cell …

The role of anionic amino acids in hydrolysis of poly-β-(1,6)-N-acetylglucosamine exopolysaccharides by the biofilm dispersing glycosidase Dispersin B

AP Breslawec, S Wang, C Li, MB Poulin - bioRxiv, 2020 - biorxiv.org
Abstract The exopolysaccharide poly-β-(1→ 6)-N-acetylglucosamine (PNAG) is a major
structural determinant of bacterial biofilms responsible for persistent and nosocomial …

Investigations of Substrate Recognition of the Biofilm Glycosidase Enzyme Dispersin B

AB Peterson - 2022 - search.proquest.com
Bacterial biofilms, which are comprised of bacterial cells embedded in a thick extracellular
polymeric substance (EPS), is a type of survival mechanism used by a variety of medically …