Whereas mechanisms underlying the fidelity of DNA polymerases (DNAPs) have been
investigated in detail, RNA polymerase (RNAP) fidelity mechanisms remained poorly …

Bacteriophage T7 RNA polymerase (RNAP) is the best characterized member of a
widespread family of RNAPs that includes most bacteriophageencoded RNAPs as well as …

Binding of a ribonucleoside triphosphate to an RNA polymerase II transcribing complex, with
base pairing to the template DNA, was revealed by X-ray crystallography. Binding of a …

Abstract A T7 RNA polymerase in which Tyr639 is mutated to Phe readily utilizes 2′‐deoxy,
2′‐NH2 and 2′‐F NTPs as substrates and has been widely used to synthesize modified …

The mechanism by which nucleotide polymerases select the correct substrate is of
fundamental importance to the fidelity of DNA replication and transcription. During the …

Abstract Bacteriophage T7 RNA polymerase (T7 RNAP) is widely used for synthesizing RNA
molecules with synthetic modifications and unnatural base pairs (UBPs) for a variety of …

Modified RNA and DNA molecules have novel properties that their natural counterparts do
not possess, such as better resistance to degradation in cells and improved pharmacokinetic …

Positive-strand RNA viruses exist as a quasi-species due to the incorporation of mutations
into the viral genome during replication by the virus-encoded RNA-dependent RNA …

We have constructed a structural model for poliovirus RNA-dependent RNA polymerase (3D
pol) in complex with a primer-template (sym/sub) and ATP. Residues found in conserved …

On average, mutations are deleterious to proteins. Mutations conferring new function to a
protein often come at the expense of protein folding or stability, reducing overall activity …