DNA repair pathway choices in CRISPR-Cas9-mediated genome editing
Many clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-
associated protein 9 (Cas9)-based genome editing technologies take advantage of Cas …
associated protein 9 (Cas9)-based genome editing technologies take advantage of Cas …
CRISPR-based genome editing through the lens of DNA repair
Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …
Efficient high-precision homology-directed repair-dependent genome editing by HDRobust
S Riesenberg, P Kanis, D Macak, D Wollny… - Nature …, 2023 - nature.com
Abstract Homology-directed repair (HDR), a method for repair of DNA double-stranded
breaks can be leveraged for the precise introduction of mutations supplied by synthetic DNA …
breaks can be leveraged for the precise introduction of mutations supplied by synthetic DNA …
Advances in genome editing through control of DNA repair pathways
Eukaryotic cells deploy overlapping repair pathways to resolve DNA damage.
Advancements in genome editing take advantage of these pathways to produce permanent …
Advancements in genome editing take advantage of these pathways to produce permanent …
Methods favoring homology-directed repair choice in response to CRISPR/Cas9 induced-double strand breaks
H Yang, S Ren, S Yu, H Pan, T Li, S Ge… - International journal of …, 2020 - mdpi.com
Precise gene editing is—or will soon be—in clinical use for several diseases, and more
applications are under development. The programmable nuclease Cas9, directed by a …
applications are under development. The programmable nuclease Cas9, directed by a …
DNA damage: from threat to treatment
A Carusillo, C Mussolino - Cells, 2020 - mdpi.com
DNA is the source of genetic information, and preserving its integrity is essential in order to
sustain life. The genome is continuously threatened by different types of DNA lesions, such …
sustain life. The genome is continuously threatened by different types of DNA lesions, such …
Thymidine nucleotide metabolism controls human telomere length
W Mannherz, S Agarwal - Nature genetics, 2023 - nature.com
Telomere length in humans is associated with lifespan and severe diseases, yet the genetic
determinants of telomere length remain incompletely defined. Here we performed genome …
determinants of telomere length remain incompletely defined. Here we performed genome …
A new class of medicines through DNA editing
MH Porteus - New England Journal of Medicine, 2019 - Mass Medical Soc
Clinical Genome Editing Therapeutic editing of the human genome has long been a holy
grail of genetic medicine. Different approaches are founded on a bedrock of basic laboratory …
grail of genetic medicine. Different approaches are founded on a bedrock of basic laboratory …
CRISPR-Cas9 fusion to dominant-negative 53BP1 enhances HDR and inhibits NHEJ specifically at Cas9 target sites
R Jayavaradhan, DM Pillis, M Goodman… - Nature …, 2019 - nature.com
Precise genome editing/correction of DNA double-strand breaks (DSBs) induced by
CRISPR-Cas9 by homology-dependent repair (HDR) is limited by the competing error-prone …
CRISPR-Cas9 by homology-dependent repair (HDR) is limited by the competing error-prone …
Highly efficient and marker-free genome editing of human pluripotent stem cells by CRISPR-Cas9 RNP and AAV6 donor-mediated homologous recombination
Genome editing of human pluripotent stem cells (hPSCs) provides powerful opportunities for
in vitro disease modeling, drug discovery, and personalized stem cell-based therapeutics …
in vitro disease modeling, drug discovery, and personalized stem cell-based therapeutics …