The role of more than 40 years of improvement in protein A chromatography in the growth of the therapeutic antibody industry
Protein A chromatography has been used as the mAb capture step in the majority of FDA
submissions. In this study, the performance of protein A chromatography, as indicated by …
submissions. In this study, the performance of protein A chromatography, as indicated by …
Emerging technologies for the integration and intensification of downstream bioprocesses
Downstream processing is currently the major bottleneck for bioproduct generation. In
contrast to the advances in fermentation processes, the tools used for downstream …
contrast to the advances in fermentation processes, the tools used for downstream …
[HTML][HTML] Evaluation of recent Protein A stationary phase innovations for capture of biotherapeutics
TM Pabst, J Thai, AK Hunter - Journal of Chromatography A, 2018 - Elsevier
We describe a comprehensive evaluation of 12 Protein A stationary phases for capture of
biotherapeutics. We first examine the morphological properties of the stationary phases …
biotherapeutics. We first examine the morphological properties of the stationary phases …
[HTML][HTML] Hydrodynamics of expanded bed adsorption studied through CFD-DEM
TMJ Nijssen, JT Padding, M Ottens - Chemical Engineering Science, 2023 - Elsevier
The hydrodynamics of the Expanded Bed Adsorption process is studied through simulations
combining Computational Fluid Dynamics and the Discrete Element Method. A …
combining Computational Fluid Dynamics and the Discrete Element Method. A …
Polypropylene nonwoven fabrics with conformal grafting of poly (glycidyl methacrylate) for bioseparations
Y Zheng, H Liu, PV Gurgel, RG Carbonell - Journal of membrane science, 2010 - Elsevier
Nonwovens fabrics are porous materials with great potential for use in the separation and
purification of biotherapeutic proteins and other biomolecules after proper surface activation …
purification of biotherapeutic proteins and other biomolecules after proper surface activation …
Influence of different packing methods on the hydrodynamic stability of chromatography columns
M Dorn, F Eschbach, D Hekmat… - … of Chromatography A, 2017 - Elsevier
It is well known that packing non-uniformity may cause peak asymmetry and limit the
performance of packed-bed chromatographic columns. However, understanding of the …
performance of packed-bed chromatographic columns. However, understanding of the …
Continuous bind‐and‐elute protein A capture chromatography: Optimization under process scale column constraints and comparison to batch operation
O Kaltenbrunner, L Diaz, X Hu… - Biotechnology …, 2016 - Wiley Online Library
Recently, continuous downstream processing has become a topic of discussion and
analysis at conferences while no industrial applications of continuous downstream …
analysis at conferences while no industrial applications of continuous downstream …
Modeling preparative cation exchange chromatography of monoclonal antibodies
S Hunt, T Larsen, RJ Todd - Preparative chromatography for …, 2017 - Wiley Online Library
Recent advances in computational power, scientific understanding, and analytical
capabilities are enabling more mechanistic modeling in biotechnology. This chapter …
capabilities are enabling more mechanistic modeling in biotechnology. This chapter …
[HTML][HTML] Chromatographic characterization and process performance of column-packed anion exchange fibrous adsorbents for high throughput and high capacity …
Fibrous materials are prominent among novel chromatographic supports for the separation
and purification of biomolecules. In this work, strong anion exchange, quaternary ammonium …
and purification of biomolecules. In this work, strong anion exchange, quaternary ammonium …
[HTML][HTML] Pressure-Flow experiments, packing, and modeling for scale-up of a mixed mode chromatography column for biopharmaceutical manufacturing
J Prentice, ST Evans, D Robbins, G Ferreira - Journal of Chromatography A, 2020 - Elsevier
To obtain consistent chromatographic behavior, it is important to develop resin packing
methods in accordance with the characteristics of each resin. Resins, particularly those with …
methods in accordance with the characteristics of each resin. Resins, particularly those with …