Protein A chromatography has been used as the mAb capture step in the majority of FDA
submissions. In this study, the performance of protein A chromatography, as indicated by …

Downstream processing is currently the major bottleneck for bioproduct generation. In
contrast to the advances in fermentation processes, the tools used for downstream …

We describe a comprehensive evaluation of 12 Protein A stationary phases for capture of
biotherapeutics. We first examine the morphological properties of the stationary phases …

The hydrodynamics of the Expanded Bed Adsorption process is studied through simulations
combining Computational Fluid Dynamics and the Discrete Element Method. A …

Nonwovens fabrics are porous materials with great potential for use in the separation and
purification of biotherapeutic proteins and other biomolecules after proper surface activation …

It is well known that packing non-uniformity may cause peak asymmetry and limit the
performance of packed-bed chromatographic columns. However, understanding of the …

Recently, continuous downstream processing has become a topic of discussion and
analysis at conferences while no industrial applications of continuous downstream …

Recent advances in computational power, scientific understanding, and analytical
capabilities are enabling more mechanistic modeling in biotechnology. This chapter …

Fibrous materials are prominent among novel chromatographic supports for the separation
and purification of biomolecules. In this work, strong anion exchange, quaternary ammonium …

To obtain consistent chromatographic behavior, it is important to develop resin packing
methods in accordance with the characteristics of each resin. Resins, particularly those with …