To elucidate principles operating in native biological systems and to develop novel
biotechnologies, synthetic biology aims to build and integrate synthetic gene circuits within …

Recombinant antibodies are rapidly developing therapeutic agents; approximately 40 novel
antibody molecules enter clinical trials each year, most of which are produced from Chinese …

Human induced pluripotent stem cells (iPSCs) are promising in regenerative medicine.
However, the risks of teratoma formation and the overgrowth of the transplanted cells …

Single-cell technologies are offering unparalleled insight into complex biology, revealing the
behavior of rare cell populations that are masked in bulk population analyses. One current …

Human induced pluripotent stem cells (hiPSC) hold promise for regenerative therapies,
though there are several safety concerns including the risk of oncogenic transformation or …

Rationale: Hereditary pulmonary alveolar proteinosis (hPAP) caused by granulocyte–
macrophage colony–stimulating factor (GM-CSF) receptor α-chain (CSF2RA) deficiency is a …

Epigenetic silencing of transgene expression represents a major obstacle for the efficient
genetic modification of multipotent and pluripotent stem cells. We and others have …

Cell-cell interactions govern differentiation and cell competition in pluripotent cells during
early development, but the investigation of such processes is hindered by a lack of efficient …

The isolation of stably transfected cell lines suitable for the manufacture of biotherapeutic
protein products can be an arduous process relying on the identification of a high …

Correction of patient-specific induced pluripotent stem cells (iPSC) upon gene delivery
through retroviral vectors offers new treatment perspectives for monogenetic diseases. Gene …