To elucidate principles operating in native biological systems and to develop novel
biotechnologies, synthetic biology aims to build and integrate synthetic gene circuits within …

Recombinant proteins used in biomedical research, diagnostics and different therapies are
mostly produced in Chinese hamster ovary cells in the pharmaceutical industry. These …

Conventional genome engineering with CRISPR–Cas9 creates double-strand breaks
(DSBs) that lead to undesirable byproducts and reduce product purity. Here we report an …

Genome sequencing studies have identified millions of somatic variants in cancer, but it
remains challenging to predict the phenotypic impact of most. Experimental approaches to …

The study of cellular processes occurring inside intact organisms requires methods to
visualize cellular functions such as gene expression in deep tissues. Ultrasound is a widely …

Multiplex genetic assays can simultaneously test thousands of genetic variants for a property
of interest. However, limitations of existing multiplex assay methods in cultured mammalian …

This review describes key milestones related to the production of biopharmaceuticals—
therapies manufactured using recombinant DNA technology. The market for …

The lack of functional evidence for the majority of missense variants limits their clinical
interpretability and poses a key barrier to the broad utility of carrier screening. In Lynch …

Plant transformation remains the most sought-after technology for functional genomics and
crop genetic improvement, especially for introducing specific new traits and to modify or …

Synthetic biology has the potential to bring forth advanced genetic devices for applications
in healthcare and biotechnology. However, accurately predicting the behavior of engineered …