[HTML][HTML] Plant molecular farming for the production of valuable proteins–Critical evaluation of achievements and future challenges

S Schillberg, R Finnern - Journal of plant physiology, 2021 - Elsevier
Recombinant proteins play an important role in many areas of our lives. For example,
recombinant enzymes are used in the food and chemical industries and as high-quality …

Putting the spotlight back on plant suspension cultures

RB Santos, R Abranches, R Fischer, M Sack… - Frontiers in plant …, 2016 - frontiersin.org
Plant cell suspension cultures have several advantages that make them suitable for the
production of recombinant proteins. They can be cultivated under aseptic conditions using …

Improving protein quantity and quality—The next level of plant molecular farming

H Liu, MP Timko - International Journal of Molecular Sciences, 2022 - mdpi.com
Plants offer several unique advantages in the production of recombinant pharmaceuticals for
humans and animals. Although numerous recombinant proteins have been expressed in …

Simulation and optimization of nutrient uptake and biomass formation using a multi-parameter Monod-type model of tobacco BY-2 cell suspension cultures in a stirred …

H Nausch, M Baldan, K Teichert, J Lutz… - Frontiers in Plant …, 2023 - frontiersin.org
Introduction Tobacco (Nicotiana tabacum) cv Bright Yellow-2 (BY-2) cell suspension cultures
enable the rapid production of complex protein-based biopharmaceuticals but currently …

Correlation between mass transfer coefficient kLa and relevant operating parameters in cylindrical disposable shaken bioreactors on a bench-to-pilot scale

W Klöckner, R Gacem, T Anderlei, N Raven… - Journal of biological …, 2013 - Springer
Background Among disposable bioreactor systems, cylindrical orbitally shaken bioreactors
show important advantages. They provide a well-defined hydrodynamic flow combined with …

Molecular farming in tobacco hairy roots by triggering the secretion of a pharmaceutical antibody

ST Häkkinen, N Raven, M Henquet… - Biotechnology and …, 2014 - Wiley Online Library
Recombinant pharmaceutical proteins expressed in hairy root cultures can be secreted into
the medium to improve product homogeneity and to facilitate purification, although this may …

Recombinant protein production in plants: A brief overview of strengths and challenges

S Schillberg, H Spiegel - Recombinant Proteins in Plants: Methods and …, 2022 - Springer
The first recombinant proteins were produced in microbes and animal cells cultivated in
bioreactors. These systems have become the standard for industrial-scale recombinant …

Optimization of BY‐2 cell suspension culture medium for the production of a human antibody using a combination of fractional factorial designs and the response …

N Vasilev, U Grömping, A Lipperts… - Plant biotechnology …, 2013 - Wiley Online Library
We have developed a strategy for the optimization of plant cell suspension culture media
using a combination of fractional factorial designs (FFD s) and response surface …

The cream of the crop: Biology, breeding and applications of Cannabis sativa

S Schilling, CA Dowling, J Shi, L Ryan, D Hunt… - Authorea …, 2020 - authorea.com
Cannabis sativa is an extraordinarily versatile species. Hemp and its cousin marijuana, both
C. sativa, have been used for millennia as a source of fibre, oil and for medicinal, spiritual …

Intracellular trafficking and glycosylation of hydroxyproline-O-glycosylation module in tobacco BY-2 cells is dependent on medium composition and transcriptome …

U Karki, P Perez Sanchez, S Chakraborty, B Dickey… - Scientific Reports, 2023 - nature.com
Expression of recombinant proteins in plant cells with a “designer” hydroxyproline (Hyp)-O-
glycosylated peptide (HypGP), such as tandem repeats of a “Ser-Pro” motif, has been shown …