Microbial bioremediation has gained attention as a cheap, efficient, and sustainable
technology to manage the increasing environmental pollution. Since microorganisms in …

Non-model bacteria like Pseudomonas putida, Lactococcus lactis and other species have
unique and versatile metabolisms, offering unique opportunities for Synthetic Biology …

Continuous evolution can generate biomolecules for synthetic biology and enable
evolutionary investigation. The orthogonal DNA replication system (OrthoRep) in yeast can …

Engineered RNA elements are programmable tools capable of detecting small molecules,
proteins, and nucleic acids. Predicting the behavior of these synthetic biology components …

Summary mRNAs can fold into complex structures that regulate gene expression. Resolving
such structures de novo has remained challenging and has limited our understanding of the …

Expanding and reprogramming the genetic code of cells for the incorporation of multiple
distinct non-canonical amino acids (ncAAs), and the encoded biosynthesis of non-canonical …

Orthogonal (O) ribosome-mediated translation of O-mRNAs enables the incorporation of up
to three distinct non-canonical amino acids (ncAAs) into proteins in Escherichia coli (E. coli) …

Gene expression models greatly accelerate the engineering of synthetic metabolic pathways
and genetic circuits by predicting sequence-function relationships and reducing trial-and …

Biotin synthase (BioB) is a member of the Radical SAM superfamily of enzymes that
catalyzes the terminal step of biotin (vitamin B7) biosynthesis, in which it inserts a sulfur …

mRNA degradation is a central process that affects all gene expression levels, and yet, the
determinants that control mRNA decay rates remain poorly characterized. Here, we applied …