Review of key issues and potential strategies in bio-degradation of polyolefins

D Kong, L Wang, Y Yuan, W Xia, Z Liu, M Shi… - Bioresource …, 2024 - Elsevier
Polyolefins are the most widely used plastic product and a major contributor to white
pollution. Currently, studies on polyolefin degradation systems are mainly focused on …

Solar-powered P450 catalysis: Engineering electron transfer pathways from photosynthesis to P450s

B Agustinus, EMJ Gillam - Journal of Inorganic Biochemistry, 2023 - Elsevier
With the increasing focus on green chemistry, biocatalysis is becoming more widely used in
the pharmaceutical and other chemical industries for sustainable production of high value …

Selective carbon-hydrogen bond hydroxylation using an engineered cytochrome P450 peroxygenase

J Akter, TP Stockdale, SA Child, JHZ Lee… - Journal of Inorganic …, 2023 - Elsevier
The cytochrome P450 enzyme CYP102A1 (P450BM3) is a versatile monooxygenase
enzyme which has been adapted and engineered for multiple applications in chemical …

Engineering Peroxygenase Activity into Cytochrome P450 Monooxygenases through Modification of the Oxygen Binding Region

MN Podgorski, J Akter, LR Churchman, JB Bruning… - ACS …, 2024 - ACS Publications
Cytochrome P450 enzymes (CYPs) are biocatalysts for the generation of fine chemicals
including natural products, drug metabolites, and flavor and fragrance compounds …

Efficient regio-and stereo-selective C–H bond hydroxylation of steroids using an engineered heme-thiolate peroxygenase biocatalyst

J Akter, EF Hayball, SG Bell - Catalysis Science & Technology, 2023 - pubs.rsc.org
A crucial reaction in chemical synthesis is the activation of unactivated carbon–hydrogen
bonds in complex molecules. We demonstrate the regio-and stereo-selective hydroxlation of …

An In Crystallo Reaction with an Engineered Cytochrome P450 Peroxygenase

JHZ Lee, JB Bruning, SG Bell - Chemistry–A European Journal, 2024 - Wiley Online Library
The cytochrome P450 monooxygenases (CYPs) are a class of heme‐thiolate enzymes that
insert oxygen into unactivated C− H bonds. These enzymes can be converted into …

[HTML][HTML] Characterisation of the heme aqua-ligand coordination environment in an engineered peroxygenase cytochrome P450 variant

MN Podgorski, JHZ Lee, JS Harbort… - Journal of Inorganic …, 2023 - Elsevier
The cytochrome P450 enzymes (CYPs) are heme-thiolate monooxygenases that catalyse
the insertion of an oxygen atom into the C–H bonds of organic molecules. In most CYPs, the …

The catalytic activity and structure of the lipid metabolizing CYP124 cytochrome P450 enzyme from Mycobacterium marinum

A Ghith, JB Bruning, SG Bell - Archives of Biochemistry and Biophysics, 2023 - Elsevier
The CYP124 family of cytochrome P450 enzymes, as exemplified by CYP124A1 from
Mycobacterium tuberculosis, is involved in the metabolism of methyl branched lipids and …

Alternative electron sources for cytochrome P450s catalytic cycle: biosensing and biosynthetic application

VV Shumyantseva, PI Koroleva, TV Bulko… - Processes, 2023 - mdpi.com
The functional significance of cytochrome P450s (CYP) enzymes is their ability to catalyze
the biotransformation of xenobiotics and endogenous compounds. P450 enzymes catalyze …

Cytochrome P450-catalyzed oxidation of halogen-containing substrates

T Coleman, MN Podgorski, ML Doyle… - Journal of Inorganic …, 2023 - Elsevier
Cytochrome P450 (CYP) enzymes are heme-thiolate monooxygenases which catalyze the
oxidation of aliphatic and aromatic CH bonds and other reactions. The oxidation of halogens …