Optimization of chemically defined cell culture media–replacing fetal bovine serum in mammalian in vitro methods
J Van der Valk, D Brunner, K De Smet… - Toxicology in vitro, 2010 - Elsevier
Quality assurance is becoming increasingly important. Good laboratory practice (GLP) and
good manufacturing practice (GMP) are now established standards. The biomedical field …
good manufacturing practice (GMP) are now established standards. The biomedical field …
[HTML][HTML] Regulatory mechanisms of Na+/glucose cotransporters in renal proximal tubule cells
YJ Lee, HJ Han - Kidney International, 2007 - Elsevier
Glucose is a key fuel and an important metabolic substrate in mammals. Renal proximal
tubular cells (PTCs) not only reabsorb filtered glucose but are also believed to play a role in …
tubular cells (PTCs) not only reabsorb filtered glucose but are also believed to play a role in …
Alternatives to the use of fetal bovine serum: serum-free cell culture
G Gstraunthaler - ALTEX-Alternatives to animal experimentation, 2003 - altex.org
Serum is commonly used as a supplement to cell culture media. It provides a broad
spectrum of macromolecules, carrier proteins for lipoid substances and trace elements …
spectrum of macromolecules, carrier proteins for lipoid substances and trace elements …
The humane collection of fetal bovine serum and possibilities for serum-free cell and tissue culture
J Van der Valk, D Mellor, R Brands, R Fischer… - Toxicology in vitro, 2004 - Elsevier
Fetal bovine serum (FBS) is a common supplement to in vitro culture media. A workshop
was organized to discuss whether or not fetuses might suffer when blood is withdrawn, and …
was organized to discuss whether or not fetuses might suffer when blood is withdrawn, and …
[图书][B] Fundamentals of Oncology, revised and expanded
HC Pitot - 2002 - taylorfrancis.com
Compiling significant advances from more than a decade of research, this text presents
breakthroughs in the prevention, treatment, and control of cancer-exploring the origin …
breakthroughs in the prevention, treatment, and control of cancer-exploring the origin …
ECVAM good cell culture practice task force report 1
T Hartung, M Balls, C Bardouille… - Alternatives to …, 2002 - journals.sagepub.com
Background The maintenance of high standards is fundamental to all good scientific
practice, and is essential for ensuring the reproducibility, reliability, credibility, acceptance …
practice, and is essential for ensuring the reproducibility, reliability, credibility, acceptance …
Examining the sources of variability in cell culture media used for biopharmaceutical production
N McGillicuddy, P Floris, S Albrecht, J Bones - Biotechnology letters, 2018 - Springer
Raw materials, in particular cell culture media, represent a significant source of variability to
biopharmaceutical manufacturing processes that can detrimentally affect cellular growth …
biopharmaceutical manufacturing processes that can detrimentally affect cellular growth …
Human serum alters cell culture behavior and improves spheroid formation in comparison to fetal bovine serum
JI Heger, K Froehlich, J Pastuschek, A Schmidt… - Experimental Cell …, 2018 - Elsevier
Background The use of fetal bovine serum (FBS) as growth supplement for human cell and
tissue culture is widely spread in basic research as well as in clinical approaches, although …
tissue culture is widely spread in basic research as well as in clinical approaches, although …
[HTML][HTML] Three‐dimensional culture of dental pulp pluripotent‐like stem cells (DPPSCs) enhances Nanog expression and provides a serum‐free condition for exosome …
FN Faruqu, S Zhou, N Sami, F Gheidari, H Lu… - FASEB …, 2020 - ncbi.nlm.nih.gov
Stem cell‐derived exosomes have been identified as novel cell‐free therapeutics for
regenerative medicine. Three‐dimensional (3D) culture of stem cells were reported to …
regenerative medicine. Three‐dimensional (3D) culture of stem cells were reported to …
Influences of extracellular matrix components on the growth and differentiation of ruminal epithelial cells in primary culture
P Galfi, G Gabel, H Martens - Research in veterinary science, 1993 - Elsevier
Primary cultures of ovine ruminal epithelial cells were made to study the influence of
collagen types I and IV and of medium supplementation with various hormones and Na-n …
collagen types I and IV and of medium supplementation with various hormones and Na-n …