Prime editing for precise and highly versatile genome manipulation

PJ Chen, DR Liu - Nature Reviews Genetics, 2023 - nature.com
Programmable gene-editing tools have transformed the life sciences and have shown
potential for the treatment of genetic disease. Among the CRISPR–Cas technologies that …

Prime editing: advances and therapeutic applications

Z Zhao, P Shang, P Mohanraju, N Geijsen - Trends in Biotechnology, 2023 - cell.com
Clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR–
Cas)-mediated genome editing has revolutionized biomedical research and will likely …

Efficient prime editing in mouse brain, liver and heart with dual AAVs

JR Davis, S Banskota, JM Levy, GA Newby… - Nature …, 2024 - nature.com
Realizing the promise of prime editing for the study and treatment of genetic disorders
requires efficient methods for delivering prime editors (PEs) in vivo. Here we describe the …

A split prime editor with untethered reverse transcriptase and circular RNA template

B Liu, X Dong, H Cheng, C Zheng, Z Chen… - Nature …, 2022 - nature.com
Delivery and optimization of prime editors (PEs) have been hampered by their large size
and complexity. Although split versions of genome-editing tools can reduce construct size …

Precise genomic editing of pathogenic mutations in RBM20 rescues dilated cardiomyopathy

T Nishiyama, Y Zhang, M Cui, H Li… - Science translational …, 2022 - science.org
Mutations in RNA binding motif protein 20 (RBM20) are a common cause of familial dilated
cardiomyopathy (DCM). Many RBM20 mutations cluster within an arginine/serine-rich (RS …

In vivo prime editing of a metabolic liver disease in mice

D Böck, T Rothgangl, L Villiger, L Schmidheini… - Science translational …, 2022 - science.org
Prime editing is a highly versatile CRISPR-based genome editing technology that works
without DNA double-strand break formation. Despite rapid technological advances, in vivo …

Designing and executing prime editing experiments in mammalian cells

JL Doman, AA Sousa, PB Randolph, PJ Chen… - Nature protocols, 2022 - nature.com
Prime editing (PE) is a precision gene editing technology that enables the programmable
installation of substitutions, insertions and deletions in cells and animals without requiring …

Delivery of CRISPR-Cas tools for in vivo genome editing therapy: Trends and challenges

EA Taha, J Lee, A Hotta - Journal of Controlled Release, 2022 - Elsevier
The discovery of clustered regularly interspaced short palindromic repeats (CRISPR)
genome editing technology opened the door to provide a versatile approach for treating …

CRISPR-based genome editing through the lens of DNA repair

TS Nambiar, L Baudrier, P Billon, A Ciccia - Molecular cell, 2022 - cell.com
Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …

Engineered virus-like particles for transient delivery of prime editor ribonucleoprotein complexes in vivo

M An, A Raguram, SW Du, S Banskota, JR Davis… - Nature …, 2024 - nature.com
Prime editing enables precise installation of genomic substitutions, insertions and deletions
in living systems. Efficient in vitro and in vivo delivery of prime editing components, however …