Transthyretin (TTR) is an essential transporter of a thyroid hormone and a holo-retinol
binding protein, found abundantly in human plasma and cerebrospinal fluid. In addition, this …

Alzheimer's disease is associated with the deposition of the amyloid-β peptide (Aβ) into
extracellular senile plaques in the brain. In vitro and in vivo observations have indicated that …

Overexpression of recombinant proteins in Escherichia coli results in misfolded and non-
active protein aggregates in the cytoplasm, so-called inclusion bodies (IB). In recent years, a …

Intrinsic tryptophan fluorescence spectroscopy is an important tool for examining the effects
of molecular crowding and confinement on the structure, dynamics, and function of proteins …

Thermal shift assays measure the stability of macromolecules and macromolecular
assemblies as a function of temperature. The Particle Stability Thermal Release Assay …

P-glycoprotein (Pgp) plays a pivotal role in drug bioavailability and multi-drug resistance
development. Understanding the protein's activity and designing effective drugs require …

Bifunctional or amphoteric photoacids simultaneously present donor (acidic) and acceptor
(basic) properties making them useful tools to analyze proton transfer reactions. In protic …

Albumin is an attractive protein for the preparation of nanoparticle with possible therapeutic
applications, due to its biodegradable, nontoxic, non-immunogenic, and metabolizable …

Recently, there has been a noticeable increase in the attention given to Process Analytical
Technology (PAT) within biopharmaceutical manufacturing. Specifically, there has been a …

The efficacy of many cancer drugs is hindered by P-glycoprotein (Pgp), a cellular pump that
removes drugs from cells. To improve chemotherapy, drugs capable of evading Pgp must be …