Revolutionizing DNA repair research and cancer therapy with CRISPR–Cas screens

SW Awwad, A Serrano-Benitez, JC Thomas… - … Reviews Molecular Cell …, 2023 - nature.com
All organisms possess molecular mechanisms that govern DNA repair and associated DNA
damage response (DDR) processes. Owing to their relevance to human disease, most …

DNA replication: Mechanisms and therapeutic interventions for diseases

HY Song, R Shen, H Mahasin, YN Guo, DG Wang - MedComm, 2023 - Wiley Online Library
Accurate and integral cellular DNA replication is modulated by multiple replication‐
associated proteins, which is fundamental to preserve genome stability. Furthermore …

Exploring the structural landscape of DNA maintenance proteins

KB Schou, S Mandacaru, M Tahir, N Tom… - Nature …, 2024 - nature.com
Evolutionary annotation of genome maintenance (GM) proteins has conventionally been
established by remote relationships within protein sequence databases. However, often no …

Co-evolution based machine-learning for predicting functional interactions between human genes

D Stupp, E Sharon, I Bloch, M Zitnik, O Zuk… - Nature …, 2021 - nature.com
Over the next decade, more than a million eukaryotic species are expected to be fully
sequenced. This has the potential to improve our understanding of genotype and phenotype …

Mechanism of DNA unwinding by MCM8-9 in complex with HROB

A Acharya, H Bret, JW Huang, M Mütze, M Göse… - Nature …, 2024 - nature.com
HROB promotes the MCM8-9 helicase in DNA damage response. To understand how
HROB activates MCM8-9, we defined their interaction interface. We showed that HROB …

Histone chaperone ASF1 acts with RIF1 to promote DNA end joining in BRCA1-deficient cells

M Tang, Z Chen, C Wang, X Feng, N Lee… - Journal of Biological …, 2022 - ASBMB
Replication timing regulatory factor 1 (RIF1) acts downstream of p53-binding protein 53BP1
to inhibit the resection of DNA broken ends, which plays critical roles in determining the DNA …

PICKLES v3: the updated database of pooled in vitro CRISPR knockout library essentiality screens

LC Novak, J Chou, M Colic, CA Bristow… - Nucleic acids …, 2023 - academic.oup.com
Abstract PICKLES (https://pickles. hart-lab. org) is an updated web interface to a freely
available database of genome-scale CRISPR knockout fitness screens in human cell lines …

Proteomic analysis of ferroptosis pathways reveals a role of CEPT1 in suppressing ferroptosis

X Liu, Z Chen, Y Yan, F Zandkarimi, L Nie, Q Li… - Protein & …, 2024 - academic.oup.com
Ferroptosis has been recognized as a unique cell death modality driven by excessive lipid
peroxidation and unbalanced cellular metabolism. In this study, we established a protein …

Low-molecular-weight cyclin E deregulates DNA replication and damage repair to promote genomic instability in breast cancer

M Li, S Tsavachidis, F Wang, T Bui, TDT Nguyen, L Luo… - Oncogene, 2022 - nature.com
Low-molecular-weight cyclin E (LMW-E) is an N-terminus deleted (40 amino acid) form of
cyclin E detected in breast cancer, but not in normal cells or tissues. LMW-E overexpression …

Exploiting the DNA Damage Response for Prostate Cancer Therapy

TH Stracker, OI Osagie, FE Escorcia, DE Citrin - Cancers, 2023 - mdpi.com
Simple Summary Localized prostate cancer has a favorable prognosis and can be
effectively treated with radiotherapy, as well as therapies that target hormone production and …