The real-time polymerase chain reaction

M Kubista, JM Andrade, M Bengtsson… - Molecular aspects of …, 2006 - Elsevier
The scientific, medical, and diagnostic communities have been presented the most powerful
tool for quantitative nucleic acids analysis: real-time PCR [Bustin, SA, 2004. A–Z of …

mRNA, microRNA and lncRNA as novel bladder tumor markers

E Wieczorek, E Reszka - Clinica chimica acta, 2018 - Elsevier
Early detection of bladder cancer (BC) is essential for improvement of the patient's prognosis
and general survival rates. Current diagnostic methods are still limited, so new specific and …

The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

JF Huggett, CA Foy, V Benes, K Emslie… - Clinical …, 2013 - academic.oup.com
There is growing interest in digital PCR (dPCR) because technological progress makes it a
practical and increasingly affordable technology. dPCR allows the precise quantification of …

Evaluation of potential reference genes for reverse transcription-qPCR studies of physiological responses in Drosophila melanogaster

F Ponton, MP Chapuis, M Pernice, GA Sword… - Journal of insect …, 2011 - Elsevier
Drosophila melanogaster is one of the most important genetic models and techniques such
as reverse transcription quantitative real-time PCR (RT-qPCR) are being employed …

Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos

K Goossens, M Van Poucke, A Van Soom… - BMC developmental …, 2005 - Springer
Background Real-time quantitative PCR is a sensitive and very efficient technique to
examine gene transcription patterns in preimplantation embryos, in order to gain information …

Development and evaluation of canine reference genes for accurate quantification of gene expression

B Brinkhof, B Spee, J Rothuizen, LC Penning - Analytical biochemistry, 2006 - Elsevier
In determining relative gene expression by quantitative measurements of mRNA levels
using real-time quantitative PCR, internal standards such as reference genes are essential …

Development and application of multiple internal reference (housekeeper) gene assays for accurate normalisation of canine gene expression studies

IR Peters, D Peeters, CR Helps, MJ Day - Veterinary immunology and …, 2007 - Elsevier
Measurement of mRNA expression by real-time RT-PCR (QRT-PCR) has proven to be an
important and powerful tool for the investigation of the pathogenesis of inflammatory and …

[HTML][HTML] Real time PCR and importance of housekeepings genes for normalization and quantification of mRNA expression in different tissues

EL Rebouças, JJN Costa, MJ Passos… - Brazilian Archives of …, 2013 - SciELO Brasil
The aim of this review was to evaluate the importance of the real-time PCR (qRT-PCR) as a
technique for mRNA expression analysis in different tissues. Real-time PCR is widely used …

Expression of taste molecules in the upper gastrointestinal tract in humans with and without type 2 diabetes

RL Young, K Sutherland, N Pezos, SM Brierley… - Gut, 2009 - gut.bmj.com
Objective: Nutrient feedback from the small intestine modulates upper gastrointestinal
function and energy intake; however, the molecular mechanism of nutrient detection is …

[HTML][HTML] Analysis of one-step and two-step real-time RT-PCR using SuperScript III

MJ Wacker, MP Godard - Journal of biomolecular techniques: JBT, 2005 - ncbi.nlm.nih.gov
Real-time reverse transcription polymerase chain reaction (RT-PCR) is a commonly used
technique to analyze gene expression. There has been little research conducted to test if …