Escherichia coli is one of the organisms of choice for the production of recombinant proteins.
Its use as a cell factory is well-established and it has become the most popular expression …

Escherichia coli is the most widely used heterologous protein expression system. However,
this system remains a challenge due to the low solubility of proteins, insufficient yield, and …

Potassium channels are responsible for the selective permeation of K+ ions across cell
membranes. K+ ions permeate in single file through the selectivity filter, a narrow pore lined …

Prokaryotic expression systems are superior in producing valuable recombinant proteins,
enzymes and therapeutic products. Conventional microbial technology is evolving gradually …

Membrane proteins are still heavily under-represented in the protein data bank (PDB),
owing to multiple bottlenecks. The typical low abundance of membrane proteins in their …

Highlights•Compares and contrasts new affinity methods to dominant conventional
methods.•Explains how the popular His tag, despite its age, still dominates this …

The production of membrane proteins of high purity and in satisfactory yields is crucial for
biomedical research. Due to their involvement in various cellular processes, membrane …

The production of recombinant proteins is one of the most significant achievements of
biotechnology in the last century. These proteins are produced in the eukaryotic or …

Advancements in peptide fusion technologies to maximize the protein production has taken
a big leap to fulfill the demands of post-genomics era targeting elucidation of …

Background Heterologous protein production in Escherichia coli often suffers from
bottlenecks such as proteolytic degradation, complex purification procedures and toxicity …