Research opportunities and techniques are reviewed for the application of hard x-ray pulsed
free-electron lasers (XFEL) to structural biology. These include the imaging of protein …

The opening of hard X-ray free-electron laser facilities, such as the Linac Coherent Light
Source (LCLS) at SLAC National Accelerator Laboratory in the United States, has ushered …

A Brownian machine, a tiny device buffeted by the random motions of molecules in the
environment, is capable of exploiting these thermal motions for many of the conformational …

The structural dynamics of a molecule are determined by the underlying potential energy
landscape. Conical intersections are funnels connecting otherwise separate potential …

Cryo-electron microscopy (CryoEM) has become a vital technique in structural biology. It is
an interdisciplinary field that takes advantage of advances in biochemistry, physics, and …

We use extremely bright and ultrashort pulses from an x-ray free-electron laser (XFEL) to
measure correlations in x rays scattered from individual bioparticles. This allows us to go …

Time‐resolved macromolecular crystallography unifies structure determination with
chemical kinetics, since the structures of transient states and chemical and kinetic …

Single particle diffractive imaging data from Rice Dwarf Virus (RDV) were recorded using the
Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS). RDV …

Single-particle imaging (SPI) with X-ray free-electron lasers has the potential to change
fundamentally how biomacromolecules are imaged. The structure would be derived from …

Using a manifold-based analysis of experimental diffraction snapshots from an X-ray free
electron laser, we determine the three-dimensional structure and conformational landscape …