X-ray lasers for structural and dynamic biology

JCH Spence, U Weierstall… - Reports on Progress in …, 2012 - iopscience.iop.org
Research opportunities and techniques are reviewed for the application of hard x-ray pulsed
free-electron lasers (XFEL) to structural biology. These include the imaging of protein …

Molecular imaging using x-ray free-electron lasers

A Barty, J Küpper, HN Chapman - Annual review of physical …, 2013 - annualreviews.org
The opening of hard X-ray free-electron laser facilities, such as the Linac Coherent Light
Source (LCLS) at SLAC National Accelerator Laboratory in the United States, has ushered …

Trajectories of the ribosome as a Brownian nanomachine

A Dashti, P Schwander, R Langlois… - Proceedings of the …, 2014 - National Acad Sciences
A Brownian machine, a tiny device buffeted by the random motions of molecules in the
environment, is capable of exploiting these thermal motions for many of the conformational …

Few-fs resolution of a photoactive protein traversing a conical intersection

A Hosseinizadeh, N Breckwoldt, R Fung, R Sepehr… - Nature, 2021 - nature.com
The structural dynamics of a molecule are determined by the underlying potential energy
landscape. Conical intersections are funnels connecting otherwise separate potential …

Emerging themes in CryoEM─ Single particle analysis image processing

JL Vilas, JM Carazo, COS Sorzano - Chemical Reviews, 2022 - ACS Publications
Cryo-electron microscopy (CryoEM) has become a vital technique in structural biology. It is
an interdisciplinary field that takes advantage of advances in biochemistry, physics, and …

Correlations in scattered X-ray laser pulses reveal nanoscale structural features of viruses

RP Kurta, JJ Donatelli, CH Yoon, P Berntsen… - Physical review …, 2017 - APS
We use extremely bright and ultrashort pulses from an x-ray free-electron laser (XFEL) to
measure correlations in x rays scattered from individual bioparticles. This allows us to go …

Mix and inject: reaction initiation by diffusion for time‐resolved macromolecular crystallography

M Schmidt - Advances in Condensed Matter Physics, 2013 - Wiley Online Library
Time‐resolved macromolecular crystallography unifies structure determination with
chemical kinetics, since the structures of transient states and chemical and kinetic …

Coherent diffraction of single Rice Dwarf virus particles using hard X-rays at the Linac Coherent Light Source

A Munke, J Andreasson, A Aquila, S Awel, K Ayyer… - Scientific data, 2016 - nature.com
Single particle diffractive imaging data from Rice Dwarf Virus (RDV) were recorded using the
Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS). RDV …

[PDF][PDF] Dragonfly: an implementation of the expand–maximize–compress algorithm for single-particle imaging

K Ayyer, TY Lan, V Elser, ND Loh - Journal of applied …, 2016 - journals.iucr.org
Single-particle imaging (SPI) with X-ray free-electron lasers has the potential to change
fundamentally how biomacromolecules are imaged. The structure would be derived from …

Conformational landscape of a virus by single-particle X-ray scattering

A Hosseinizadeh, G Mashayekhi, J Copperman… - Nature …, 2017 - nature.com
Using a manifold-based analysis of experimental diffraction snapshots from an X-ray free
electron laser, we determine the three-dimensional structure and conformational landscape …