Ultra-high-performance liquid chromatography high-resolution mass spectrometry variants for metabolomics research

L Perez de Souza, S Alseekh, F Scossa, AR Fernie - Nature Methods, 2021 - nature.com
Ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC–
HRMS) variants currently represent the best tools to tackle the challenges of complexity and …

Genetic engineering of microalgae for enhanced lipid production

CF Muñoz, C Südfeld, MIS Naduthodi… - Biotechnology …, 2021 - Elsevier
Microalgae have the potential to become microbial cell factories for lipid production. Their
ability to convert sunlight and CO 2 into valuable lipid compounds has attracted interest from …

Real-time bioelectronic sensing of environmental contaminants

JT Atkinson, L Su, X Zhang, GN Bennett, JJ Silberg… - Nature, 2022 - nature.com
Real-time chemical sensing is crucial for applications in environmental and health
monitoring. Biosensors can detect a variety of molecules through genetic circuits that use …

Lipid production in Yarrowia lipolytica is maximized by engineering cytosolic redox metabolism

K Qiao, TM Wasylenko, K Zhou, P Xu… - Nature …, 2017 - nature.com
Microbial factories have been engineered to produce lipids from carbohydrate feedstocks for
production of biofuels and oleochemicals. However, even the best yields obtained to date …

[HTML][HTML] Flavin-based electron bifurcation, ferredoxin, flavodoxin, and anaerobic respiration with protons (Ech) or NAD+ (Rnf) as electron acceptors: a historical review

W Buckel, RK Thauer - Frontiers in microbiology, 2018 - frontiersin.org
Flavin-based electron bifurcation is a newly discovered mechanism, by which a hydride
electron pair from NAD (P) H, coenzyme F 420 H 2, H 2, or formate is split by flavoproteins …

[HTML][HTML] Review of NAD (P) H-dependent oxidoreductases: Properties, engineering and application

LS Vidal, CL Kelly, PM Mordaka, JT Heap - Biochimica et Biophysica Acta …, 2018 - Elsevier
Abstract NAD (P) H-dependent oxidoreductases catalyze the reduction or oxidation of a
substrate coupled to the oxidation or reduction, respectively, of a nicotinamide adenine …

[HTML][HTML] Energy conservation via electron bifurcating ferredoxin reduction and proton/Na+ translocating ferredoxin oxidation

W Buckel, RK Thauer - Biochimica et Biophysica Acta (BBA)-Bioenergetics, 2013 - Elsevier
The review describes four flavin-containing cytoplasmatic multienzyme complexes from
anaerobic bacteria and archaea that catalyze the reduction of the low potential ferredoxin by …

[HTML][HTML] NADPH-generating systems in bacteria and archaea

SK Spaans, RA Weusthuis, J Van Der Oost… - Frontiers in …, 2015 - frontiersin.org
Reduced nicotinamide adenine dinucleotide phosphate (NADPH) is an essential electron
donor in all organisms. It provides the reducing power that drives numerous anabolic …

Discovery proteomics in aging human skeletal muscle finds change in spliceosome, immunity, proteostasis and mitochondria

C Ubaida-Mohien, A Lyashkov, M Gonzalez-Freire… - Elife, 2019 - elifesciences.org
A decline of skeletal muscle strength with aging is a primary cause of mobility loss and frailty
in older persons, but the molecular mechanisms of such decline are not understood. Here …

[HTML][HTML] Reserve flux capacity in the pentose phosphate pathway enables Escherichia coli's rapid response to oxidative stress

D Christodoulou, H Link, T Fuhrer, K Kochanowski… - Cell systems, 2018 - cell.com
To counteract oxidative stress and reactive oxygen species (ROS), bacteria evolved various
mechanisms, primarily reducing ROS through antioxidant systems that utilize cofactor …