Fueled by breakthrough technology developments, the biological, biomedical, and
behavioral sciences are now collecting more data than ever before. There is a critical need …

A fundamental challenge in biological research is achieving an atomic-level description and
mechanistic understanding of the function of biomolecules. Techniques for biomolecular …

Proteins are dynamic entities that undergo a plethora of conformational changes that may
take place on a wide range of time scales. These changes can be as small as the rotation of …

Highlights•The principles of protein structural ensemble determination are described.•The
use of experimental data averaged over multiple states is analysed.•Approaches for …

To advance microfluidic integration, we present the use of two-photon additive
manufacturing to fold 2D channel layouts into compact free-form 3D fluidic circuits with …

A grand challenge in the field of structural biology is to design and engineer proteins that
exhibit targeted functions. Although much success on this front has been achieved, design …

Highlights•SAXS captures architecture and dynamics to elucidate molecular
ensembles.•SAXS advances now probe key thermodynamic and kinetic properties of …

While protein conformational heterogeneity plays an important role in many aspects of
biological function, including ligand binding, its impact has been difficult to quantify …

Nuclear magnetic resonance (NMR) spectroscopy is a versatile tool for probing structure,
dynamics, folding, and interactions at atomic resolution. While naturally occurring …

Allostery is an inherent feature of proteins, but it remains challenging to reveal the
mechanisms by which allosteric signals propagate. A clearer understanding of this intrinsic …