Understanding and overcoming the pitfalls and biases of next-generation sequencing (NGS) methods for use in the routine clinical microbiological diagnostic …
Recent advancements in next-generation sequencing (NGS) have provided the foundation
for modern studies into the composition of microbial communities. The use of these NGS …
for modern studies into the composition of microbial communities. The use of these NGS …
Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples
Genome sequencing has become a powerful tool for studying emerging infectious diseases;
however, genome sequencing directly from clinical samples (ie, without isolation and …
however, genome sequencing directly from clinical samples (ie, without isolation and …
Diagnostic metagenomics: potential applications to bacterial, viral and parasitic infections
MJ Pallen - Parasitology, 2014 - cambridge.org
The term 'shotgun metagenomics' is applied to the direct sequencing of DNA extracted from
a sample without culture or target-specific amplification or capture. In diagnostic …
a sample without culture or target-specific amplification or capture. In diagnostic …
de novo assembly and population genomic survey of natural yeast isolates with the Oxford Nanopore MinION sequencer
Abstract Background: Oxford Nanopore Technologies Ltd (Oxford, UK) have recently
commercialized MinION, a small single-molecule nanopore sequencer, that offers the …
commercialized MinION, a small single-molecule nanopore sequencer, that offers the …
Resistance analysis of genotype 3 hepatitis C virus indicates subtypes inherently resistant to nonstructural protein 5A inhibitors
Hepatitis C virus (HCV) genotype (gt) 3 is highly prevalent globally, with non‐gt3a subtypes
common in Southeast Asia. Resistance‐associated substitutions (RASs) have been shown …
common in Southeast Asia. Resistance‐associated substitutions (RASs) have been shown …
High-throughput whole genome sequencing of Porcine reproductive and respiratory syndrome virus from cell culture materials and clinical specimens using next …
Next-generation sequencing (NGS) technologies have increasingly played crucial roles in
biological and medical research, but are not yet in routine use in veterinary diagnostic …
biological and medical research, but are not yet in routine use in veterinary diagnostic …
Conservation genomics reveals pesticide and pathogen exposure in the declining bumble bee Bombus terricola
N Tsvetkov, VJ MacPhail, SR Colla… - Molecular ecology, 2021 - Wiley Online Library
In recent years, many pollinators have experienced large population declines, which
threaten food security and the stability of natural ecosystems. Bumble bees are particularly …
threaten food security and the stability of natural ecosystems. Bumble bees are particularly …
Norovirus whole-genome sequencing by SureSelect target enrichment: a robust and sensitive method
Norovirus full-genome sequencing is challenging due to sequence heterogeneity among
genomes. Previous methods have relied on PCR amplification, which is problematic due to …
genomes. Previous methods have relied on PCR amplification, which is problematic due to …
A universal protocol to generate consensus level genome sequences for foot-and-mouth disease virus and other positive-sense polyadenylated RNA viruses using …
G Logan, GL Freimanis, DJ King, B Valdazo-González… - BMC genomics, 2014 - Springer
Abstract Background Next-Generation Sequencing (NGS) is revolutionizing molecular
epidemiology by providing new approaches to undertake whole genome sequencing (WGS) …
epidemiology by providing new approaches to undertake whole genome sequencing (WGS) …
Full genome analysis of bovine astrovirus from fecal samples of cattle in Japan: identification of possible interspecies transmission of bovine astrovirus
M Nagai, T Omatsu, H Aoki, K Otomaru, T Uto… - Archives of …, 2015 - Springer
A viral metagenomics approach was used to investigate fecal samples of Japanese calves
with and without diarrhea. Of the different viral pathogens detected, read counts gave nearly …
with and without diarrhea. Of the different viral pathogens detected, read counts gave nearly …