More than 3000 type II restriction endonucleases have been discovered. They recognize
short, usually palindromic, sequences of 4–8 bp and, in the presence of Mg2+, cleave the …

Restriction endonucleases are enzymes which recognize short DNA sequences and cleave
the DNA in both strands. Depending on the enzymological properties different types are …

Thermodynamic studies have demonstrated the central importance of a large negative heat
capacity change (Δ C° assoc) in site-specific protein-DNA recognition. Dissection of the …

Publisher Summary This chapter discusses DNA and RNA cleavage by metal complexes.
DNA and RNA cleavage, a very active field of research, has been developed in two main …

The crystal structure of EcoRV endonuclease has been determined at 2.5 A resolution and
that of its complexes with the cognate DNA decamer GGGATATCCC (recognition sequence …

The crystal structure of restriction endonuclease Bam HI complexed to DNA has been
determined at 2.2 angstrom resolution. The DNA binds in the cleft and retains a B-DNA type …

The structure of a complex between DNase I and d (GCGATCGC) 2 has been solved by
molecular replacement and refined to an R-factor of 0· 174 for all data between 6 and 2 Å …

Restriction endonucleases and modification methylases offer excellent systems for studying
protein-DNA interactions. The past year has seen the experimental verification of some …

This paper considers how enzymes that catalyze reactions at specific DNA sites have been
engineered to overcome the problem of competitive inhibition by excess nonspecific binding …

Revised Manuscript Received October 27, 1994® abstract: The type II restriction
endonuclease EcoRV was crystallized as a complex with the substrate DNA undecamer …