Sequencing-based analyses of microbiomes have traditionally focused on addressing the
question of community membership and profiling taxonomic abundance through amplicon …

A transcriptome constructed from short-read RNA sequencing (RNA-seq) is an easily
attainable proxy catalog of protein-coding genes when genome assembly is unnecessary …

Background The possibility of generating large RNA-sequencing datasets has led to
development of various reference-based and de novo transcriptome assemblers with their …

Background In recent years, massively parallel complementary DNA sequencing (RNA
sequencing [RNA-Seq]) has emerged as a fast, cost-effective, and robust technology to …

It is largely unknown how living organisms—especially vertebrates—survive and thrive in
the coldness, darkness and high pressures of the hadal zone. Here, we describe the unique …

Characterizing transcriptomes in non-model organisms has resulted in a massive increase
in our understanding of biological phenomena. This boon, largely made possible via high …

Background The explosive radiation and diversification of the advanced snakes (superfamily
Colubroidea) was associated with changes in all aspects of the shared venom system …

Motivation With the decreased cost of RNA-Seq, an increasing number of non-model
organisms have been sequenced. Due to the lack of reference genomes, de novo …

Background Ecdysis is an innate behaviour programme by which all arthropods moult their
exoskeletons. The complex suite of interacting neuropeptides that orchestrate ecdysis is well …

De novo transcriptome assembly is a powerful tool, and has been widely used over the last
decade for making evolutionary inferences. However, it relies on two implicit assumptions …