Multiphysics microfluidics for cell manipulation and separation: a review

H Cha, H Fallahi, Y Dai, D Yuan, H An, NT Nguyen… - Lab on a Chip, 2022 - pubs.rsc.org
Multiphysics microfluidics, which combines multiple functional physical processes in a
microfluidics platform, is an emerging research area that has attracted increasing interest for …

Microfluidic Platforms for Real‐Time In Situ Monitoring of Biomarkers for Cellular Processes

C Lou, H Yang, Y Hou, H Huang, J Qiu… - Advanced …, 2024 - Wiley Online Library
Cellular processes are mechanisms carried out at the cellular level that are aimed at
guaranteeing the stability of the organism they comprise. The investigation of cellular …

Bacteriostatic antibiotics promote CRISPR-Cas adaptive immunity by enabling increased spacer acquisition

T Dimitriu, E Kurilovich, U Łapińska, K Severinov… - Cell host & …, 2022 - cell.com
Phages impose strong selection on bacteria to evolve resistance against viral predation.
Bacteria can rapidly evolve phage resistance via receptor mutation or using their CRISPR …

Persister Escherichia coli cells have a lower intracellular pH than susceptible cells but maintain their pH in response to antibiotic treatment

O Goode, A Smith, A Zarkan, J Cama, BM Invergo… - MBio, 2021 - Am Soc Microbiol
Persister and viable but non-culturable (VBNC) cells are two clonal subpopulations that can
survive multidrug exposure via a plethora of putative molecular mechanisms. Here, we …

Fast bacterial growth reduces antibiotic accumulation and efficacy

U Łapińska, M Voliotis, KK Lee, A Campey, MRL Stone… - Elife, 2022 - elifesciences.org
Phenotypic variations between individual microbial cells play a key role in the resistance of
microbial pathogens to pharmacotherapies. Nevertheless, little is known about cell …

Antibiotic resistance via bacterial cell shape-shifting

N Ojkic, D Serbanescu, S Banerjee - Mbio, 2022 - Am Soc Microbiol
Bacteria have evolved to develop multiple strategies for antibiotic resistance by effectively
reducing intracellular antibiotic concentrations or antibiotic binding affinities, but the role of …

Slow growing bacteria survive bacteriophage in isolation

EL Attrill, U Łapińska, ER Westra… - ISME …, 2023 - academic.oup.com
The interactions between bacteria and bacteriophage have important roles in the global
ecosystem; in turn changes in environmental parameters affect the interactions between …

New understanding of multidrug efflux and permeation in antibiotic resistance, persistence, and heteroresistance

PD Manrique, CA López, S Gnanakaran… - Annals of the New …, 2023 - Wiley Online Library
Antibiotics effective against Gram‐negative ESKAPE pathogens are a critical area of unmet
need. Infections caused by these pathogens are not only difficult to treat but finding new …

Cellulose-Derived Carbon Dots for Inner Filter Effect-Based Selective Sensing of Ofloxacin Antibiotics

R Aggarwal, AK Garg, V Kumar, H Jonwal… - ACS Applied Nano …, 2023 - ACS Publications
A two-step viable synthetic approach for quick and energy-efficient acid-based charring is
described here to fabricate self-passivated fluorescent water-soluble CD (wsCD) from …

Label-free detection of single living bacteria: single-entity electrochemistry targeting metabolic products

Q Wang, J Lin, S Li, H Tian, D Zhang, Q Xin - Analytical Chemistry, 2023 - ACS Publications
This study presents a novel approach employing single-entity electrochemistry for the label-
free detection of living Escherichia coli. By examination of the collision signals generated …