Mechanical principles of nuclear shaping and positioning

TP Lele, RB Dickinson, GG Gundersen - Journal of Cell Biology, 2018 - rupress.org
Positioning and shaping the nucleus represents a mechanical challenge for the migrating
cell because of its large size and resistance to deformation. Cells shape and position the …

The role of stiffness in cell reprogramming: a potential role for biomaterials in inducing tissue regeneration

M d'Angelo, E Benedetti, MG Tupone, M Catanesi… - Cells, 2019 - mdpi.com
The mechanotransduction is the process by which cells sense mechanical stimuli such as
elasticity, viscosity, and nanotopography of extracellular matrix and translate them into …

Multiplexed 3D atlas of state transitions and immune interaction in colorectal cancer

JR Lin, S Wang, S Coy, YA Chen, C Yapp, M Tyler… - Cell, 2023 - cell.com
Advanced solid cancers are complex assemblies of tumor, immune, and stromal cells
characterized by high intratumoral variation. We use highly multiplexed tissue imaging, 3D …

A CRISPR/Cas9-Engineered ARID1A-Deficient Human Gastric Cancer Organoid Model Reveals Essential and Nonessential Modes of Oncogenic Transformation

YH Lo, KS Kolahi, Y Du, CY Chang, A Krokhotin, A Nair… - Cancer discovery, 2021 - AACR
Mutations in ARID1A rank among the most common molecular aberrations in human cancer.
However, oncogenic consequences of ARID1A mutation in human cells remain poorly …

Graph-based autoencoder integrates spatial transcriptomics with chromatin images and identifies joint biomarkers for Alzheimer's disease

X Zhang, X Wang, GV Shivashankar, C Uhler - Nature Communications, 2022 - nature.com
Tissue development and disease lead to changes in cellular organization, nuclear
morphology, and gene expression, which can be jointly measured by spatial transcriptomic …

HP1α is a chromatin crosslinker that controls nuclear and mitotic chromosome mechanics

AR Strom, RJ Biggs, EJ Banigan, X Wang, K Chiu… - Elife, 2021 - elifesciences.org
Chromatin, which consists of DNA and associated proteins, contains genetic information and
is a mechanical component of the nucleus. Heterochromatic histone methylation controls …

Chromatin reprogramming and bone regeneration in vitro and in vivo via the microtopography-induced constriction of cell nuclei

X Wang, V Agrawal, CL Dunton, Y Liu… - Nature biomedical …, 2023 - nature.com
Topographical cues on cells can, through contact guidance, alter cellular plasticity and
accelerate the regeneration of cultured tissue. Here we show how changes in the nuclear …

Unsupervised representation learning of chromatin images identifies changes in cell state and tissue organization in DCIS

X Zhang, S Venkatachalapathy, D Paysan… - Nature …, 2024 - nature.com
Ductal carcinoma in situ (DCIS) is a pre-invasive tumor that can progress to invasive breast
cancer, a leading cause of cancer death. We generate a large-scale tissue microarray …

Effect of nuclear stiffness on cell mechanics and migration of human breast cancer cells

T Fischer, A Hayn, CT Mierke - Frontiers in cell and developmental …, 2020 - frontiersin.org
The migration and invasion of cancer cells through 3D confined extracellular matrices is
coupled to cell mechanics and the mechanics of the extracellular matrix. Cell mechanics is …

Nucleophagy: from homeostasis to disease

ME Papandreou, N Tavernarakis - Cell Death & Differentiation, 2019 - nature.com
Nuclear abnormalities are prominent in degenerative disease and progeria syndromes.
Selective autophagy of organelles is instrumental in maintaining cell homeostasis and …