Single-molecule super-resolution microscopy has developed from a specialized technique
into one of the most versatile and powerful imaging methods of the nanoscale over the past …

▪ Abstract Measurements of trajectories of individual proteins or lipids in the plasma
membrane of cells show a variety of types of motion. Brownian motion is observed, but many …

Recent advances in single-molecule detection and single-molecule spectroscopy at room
temperature by laser-induced fluorescence offer new tools for the study of individual …

Enzymatic turnovers of single cholesterol oxidase molecules were observed in real time by
monitoring the emission from the enzyme's fluorescent active site, flavin adenine …

Gene expression originates from individual DNA molecules within living cells. Like many
single-molecule processes, gene expression and regulation are stochastic, that is, sporadic …

A methodology has been developed for the study of molecular recognition at the level of
single events and for the localization of sites on biosurfaces, in combining force microscopy …

One of the most basic questions in single-molecule microscopy concerns the accuracy with
which the location of a single molecule can be determined. Using the Fisher information …

We describe a method, based on single-molecule imaging, that allows the real-time
visualization of the infection pathway of single viruses in living cells, each labeled with only …

The essential postulates of classical thermodynamics are formulated, from which the second
law is deduced as the principle of increase of entropy in irreversible adiabatic processes that …

Optical microscopes have for centuries been our window to the microscopic world. The
advent of single-molecule optics over the past few decades has ushered in a new era in …