Super-resolution microscopy with single molecules in biology and beyond–essentials, current trends, and future challenges
L Möckl, WE Moerner - Journal of the American Chemical …, 2020 - ACS Publications
Single-molecule super-resolution microscopy has developed from a specialized technique
into one of the most versatile and powerful imaging methods of the nanoscale over the past …
into one of the most versatile and powerful imaging methods of the nanoscale over the past …
Single-particle tracking: applications to membrane dynamics
MJ Saxton, K Jacobson - Annual review of biophysics and …, 1997 - annualreviews.org
▪ Abstract Measurements of trajectories of individual proteins or lipids in the plasma
membrane of cells show a variety of types of motion. Brownian motion is observed, but many …
membrane of cells show a variety of types of motion. Brownian motion is observed, but many …
Fluorescence spectroscopy of single biomolecules
S Weiss - Science, 1999 - science.org
Recent advances in single-molecule detection and single-molecule spectroscopy at room
temperature by laser-induced fluorescence offer new tools for the study of individual …
temperature by laser-induced fluorescence offer new tools for the study of individual …
Single-molecule enzymatic dynamics
Enzymatic turnovers of single cholesterol oxidase molecules were observed in real time by
monitoring the emission from the enzyme's fluorescent active site, flavin adenine …
monitoring the emission from the enzyme's fluorescent active site, flavin adenine …
Central dogma at the single-molecule level in living cells
Gene expression originates from individual DNA molecules within living cells. Like many
single-molecule processes, gene expression and regulation are stochastic, that is, sporadic …
single-molecule processes, gene expression and regulation are stochastic, that is, sporadic …
Detection and localization of individual antibody-antigen recognition events by atomic force microscopy.
P Hinterdorfer, W Baumgartner… - Proceedings of the …, 1996 - National Acad Sciences
A methodology has been developed for the study of molecular recognition at the level of
single events and for the localization of sites on biosurfaces, in combining force microscopy …
single events and for the localization of sites on biosurfaces, in combining force microscopy …
Localization accuracy in single-molecule microscopy
One of the most basic questions in single-molecule microscopy concerns the accuracy with
which the location of a single molecule can be determined. Using the Fisher information …
which the location of a single molecule can be determined. Using the Fisher information …
Real-time single-molecule imaging of the infection pathway of an adeno-associated virus
G Seisenberger, MU Ried, T Endress, H Buning… - Science, 2001 - science.org
We describe a method, based on single-molecule imaging, that allows the real-time
visualization of the infection pathway of single viruses in living cells, each labeled with only …
visualization of the infection pathway of single viruses in living cells, each labeled with only …
The physics and mathematics of the second law of thermodynamics
EH Lieb, J Yngvason - Physics Reports, 1999 - Elsevier
The essential postulates of classical thermodynamics are formulated, from which the second
law is deduced as the principle of increase of entropy in irreversible adiabatic processes that …
law is deduced as the principle of increase of entropy in irreversible adiabatic processes that …
Interferometric scattering microscopy (iSCAT): new frontiers in ultrafast and ultrasensitive optical microscopy
J Ortega-Arroyo, P Kukura - Physical Chemistry Chemical Physics, 2012 - pubs.rsc.org
Optical microscopes have for centuries been our window to the microscopic world. The
advent of single-molecule optics over the past few decades has ushered in a new era in …
advent of single-molecule optics over the past few decades has ushered in a new era in …