Cancer cells are dependent upon an abundance of ribosomes to maintain rapid cell growth
and proliferation. The rate-limiting step of ribosome biogenesis is ribosomal RNA (rRNA) …

Eukaryotic RNA polymerase II (Pol II) is an essential enzyme that lies at the core of
eukaryotic biology. Due to its pivotal role in gene expression, Pol II has been subjected to a …

Abstract RNA polymerase I (Pol I) synthesizes ribosomal RNA (rRNA), which is the first and
rate-limiting step in ribosome biosynthesis. A12. 2 (A12) is a critical subunit of Pol I that is …

Transcription is a tightly regulated, complex, and essential cellular process in all living
organisms. Transcription is comprised of three steps, transcription initiation, elongation, and …

Parameter optimization or “data fitting” is a computational process that identifies a set of
parameter values that best describe an experimental data set. Parameter optimization is …

Eukaryotes express three DNA-dependent RNA polymerases (Pols) that are responsible for
the entirety of cellular genomic expression. The three Pols have evolved to express specific …

The sequence of the DNA template has long been thought to influence the rate of
transcription by DNA-dependent RNA polymerases, but the influence of DNA sequence on …

Eukaryotes express three nuclear RNA polymerases (Pols I, II, and III) that are essential for
cell survival. Despite extensive investigation of the three Pols, significant knowledge gaps …

Here we present an optimized protocol for monitoring and analyzing single nucleotide
incorporation by RNA polymerases. This protocol describes the assembly of Saccharomyces …

Lithium-ion battery separator is one of the core battery components, owing to its assistance
in ion migration and prohibiting electron transport inside the battery. Traditional separators …