Super-resolution microscopy has overcome a long-held resolution barrier—the diffraction
limit—in light microscopy and enabled visualization of previously invisible molecular details …

Structured illumination microscopy (SIM) allows rapid, super-resolution (SR) imaging in live
specimens. We review recent technical advances in SR-SIM, with emphasis on imaging …

Structured light refers to the generation and application of custom light fields. As the tools
and technology to create and detect structured light have evolved, steadily the applications …

Fluorescence microscopy is a key driver of discoveries in the life sciences, with observable
phenomena being limited by the optics of the microscope, the chemistry of the fluorophores …

Far-field optical microscopy using focused light is an important tool in a number of scientific
disciplines including chemical,(bio) physical and biomedical research, particularly with …

Anyone who has used a light microscope has wished that its resolution could be a little
better. Now, after centuries of gradual improvements, fluorescence microscopy has made a …

Determination of the molecular architecture of synapses requires nanoscopic image
resolution and specific molecular recognition, a task that has so far defied many …

Using spatially non-uniform illumination significantly improves the resolution of light
microscopy. Indeed, frequency mixing between the object and the illumination permits the …

Using ultralow light intensities that are well suited for investigating biological samples, we
demonstrate whole-cell superresolution imaging by nonlinear structured-illumination …

Imaging membranes in live cells with nanometer-scale resolution promises to reveal
ultrastructural dynamics of organelles that are essential for cellular functions. In this work, we …