On UV irradiation of Escherichia coli cells, DNA replication is transiently arrested to allow
removal of DNA damage by DNA repair mechanisms. This is followed by a resumption of …

Microorganisms that persist in single hosts face particular challenges. Helicobacter pylori,
an obligate bacterial parasite of the human stomach, has evolved a lifestyle that features …

Abstract Trans-4-hydroxy-2-nonenal (4-HNE), a major electrophilic by-product of lipid
peroxidation, is able to interact with DNA to form exocyclic guanine adducts. 4-HNE is a …

In most bacteria, cell division depends on the tubulin homolog FtsZ and other proteins, such
as SepF, that form a complex termed the divisome. Cell division also depends on FtsZ in …

Sharlyn J. Mazur** and Lawrence Grossman* Department of Biochemistry, The Johns
Hopkins University School of Hygiene and Public Health, Baltimore, Maryland 21205 …

Mitomycin C induces both MC-mono-dG and cross-linked dG-adducts in vivo. Interstrand
cross-linked (ICL) dG-MC-dG-DNA adducts can prevent strand separation. In Escherichia …

We have sequenced and genetically characterized comF, a Bacillus subtilis competence
locus, previously identified by Tn917 transposon insertion mutagenesis. Expression of the …

To better define the molecular architecture of nucleotide excision repair intermediates it is
necessary to identify the specific domains of UvrA, UvrB, and UvrC that are in close proximity …

During the process of E. coli nucleotide excision repair, DNA damage recognition and
processing are achieved by the action of the uvrA, uvrB, and uvrC gene products. The …

We have developed a new helicase assay that overcomes many limitations of other assays
used to measure this activity. This continuous, kinetic assay is based on the displacement of …