Better Cryo-EM specimen preparation: how to deal with the air–water Interface?

N Liu, HW Wang - Journal of Molecular Biology, 2023 - Elsevier
Cryogenic electron microscopy (cryo-EM) is now one of the most powerful and widely used
methods to determine high-resolution structures of macromolecules. A major bottleneck of …

Graphene Membranes for Multi‐Dimensional Electron Microscopy Imaging: Preparation, Application, and Prospect

X Gao, L Zheng, Y Yao, H Peng - Advanced Functional …, 2022 - Wiley Online Library
Technological breakthrough in electron microscopy (EM) has started a resolution revolution
in EM imaging. Nowadays, the promotion of resolution demands a robust background‐noise …

Graphene sandwich–based biological specimen preparation for cryo-EM analysis

J Xu, X Gao, L Zheng, X Jia, K Xu… - Proceedings of the …, 2024 - National Acad Sciences
High-quality specimen preparation plays a crucial role in cryo-electron microscopy (cryo-
EM) structural analysis. In this study, we have developed a reliable and convenient …

Dual-affinity graphene sheets for high-resolution cryo-electron microscopy

H Cheng, L Zheng, N Liu, C Huang, J Xu… - Journal of the …, 2023 - ACS Publications
With the development of cryo-electron microscopy (cryo-EM), high-resolution structures of
macromolecules can be reconstructed by the single particle method efficiently. However …

Optimizing selectivity via membrane molecular packing manipulation for simultaneous cation and anion screening

QW Meng, J Li, Z Lai, W Xian, S Wang, F Chen… - Science …, 2024 - science.org
Advancing membranes with enhanced solute-solute selectivity is essential for expanding
membrane technology applications, yet it presents a notable challenge. Drawing inspiration …

Nanostructured High Entropy Alloys as Structural and Functional Materials

W Zhu, X Gao, Y Yao, S Hu, Z Li, Y Teng, H Wang… - ACS …, 2024 - ACS Publications
Since their introduction in 2004, high entropy alloys (HEAs) have attracted significant
attention due to their exceptional mechanical and functional properties. Advances in our …

Graphene wettability: Fundamentals, modulations, and applications in energy fields

Y Huang, B Mao, H Li, J Zhang - Materials Chemistry and Physics, 2024 - Elsevier
Over the last decade, there has been a remarkable surge in the development of graphene-
based energy generation, storage, transduction, and harvesting devices, driven by the …

[HTML][HTML] Graphene in cryo-EM specimen optimization

N Liu, HW Wang - Current Opinion in Structural Biology, 2024 - Elsevier
Specimen preparation is a critical but challenging step in high-resolution cryogenic electron
microscopy (cryo-EM) structural analysis of macromolecules. In the past decade, graphene …

[HTML][HTML] Precision defect integrated graphene as reliable support membrane for high-resolution cryo-transmission electron microscopy

K Sharma, U López-Sánchez, H Nury, G Schoehn… - Carbon, 2024 - Elsevier
Graphene is an excellent support film for high-resolution transmission electron microscopy
(TEM) but its use with biological samples, notably in cryo-TEM, is hindered by its inherent …

Structure of the Spring Viraemia of Carp Virus Ribonucleoprotein Complex Reveals Its Assembly Mechanism and Application in Antiviral Drug Screening

ZX Wang, B Liu, T Yang, D Yu, C Zhang… - Journal of …, 2023 - Am Soc Microbiol
Spring viremia of carp virus (SVCV) is a highly pathogenic Vesiculovirus infecting the
common carp, yet neither a vaccine nor effective therapies are available to treat spring …