piggyBac-ing models and new therapeutic strategies

LE Woodard, MH Wilson - Trends in biotechnology, 2015 - cell.com
DNA transposons offer an efficient nonviral method of permanently modifying the genomes
of mammalian cells. The piggyBac transposon system has proven effective in genomic …

[HTML][HTML] Human iPSC-derived natural killer cells engineered with chimeric antigen receptors enhance anti-tumor activity

Y Li, DL Hermanson, BS Moriarity, DS Kaufman - Cell stem cell, 2018 - cell.com
Chimeric antigen receptors (CARs) significantly enhance the anti-tumor activity of immune
effector cells. Although most studies have evaluated CAR expression in T cells, here we …

[HTML][HTML] Development of innate immune cells from human pluripotent stem cells

D Bernareggi, S Pouyanfard, DS Kaufman - Experimental hematology, 2019 - Elsevier
Mouse and human pluripotent stem cells have been widely used to study the development
of the hematopoietic and immune systems. Although not all cells can be derived with the …

Rapid lentiviral vector producer cell line generation using a single DNA construct

YH Chen, C Pallant, CJ Sampson, A Boiti… - … Therapy Methods & …, 2020 - cell.com
Stable suspension producer cell lines for the production of vesicular stomatitis virus
envelope glycoprotein (VSVg)-pseudotyped lentiviral vectors represent an attractive …

Highly multiplexed genome engineering using CRISPR/Cas9 gRNA arrays

M Kurata, NK Wolf, WS Lahr, MT Weg, MG Kluesner… - PLoS one, 2018 - journals.plos.org
The CRISPR/Cas9 system is an RNA guided nuclease system that evolved as a mechanism
of adaptive immunity in bacteria. This system has been adopted for numerous genome …

Concise review: human pluripotent stem cells to produce cell-based cancer immunotherapy

H Zhu, YS Lai, Y Li, RH Blum, DS Kaufman - Stem Cells, 2018 - academic.oup.com
Human pluripotent stem cells (PSCs) provide a promising resource to produce immune cells
for adoptive cellular immunotherapy to better treat and potentially cure otherwise lethal …

Modified cells and methods of therapy

B Moriarity, B Webber, M Choudhry, RS McIvor… - US Patent …, 2021 - Google Patents
US11147837B2 - Modified cells and methods of therapy - Google Patents US11147837B2 -
Modified cells and methods of therapy - Google Patents Modified cells and methods of therapy …

Simple and efficient methods for enrichment and isolation of endonuclease modified cells

BS Moriarity, EP Rahrmann, DA Beckmann… - PloS one, 2014 - journals.plos.org
The advent of Transcription Activator-Like Effector Nucleases (TALENs), and similar
technologies such as CRISPR, provide a straightforward and cost effective option for …

Using RNA-seq and targeted nucleases to identify mechanisms of drug resistance in acute myeloid leukemia

SK Rathe, BS Moriarity, CB Stoltenberg, M Kurata… - Scientific reports, 2014 - nature.com
The evolution from microarrays to transcriptome deep-sequencing (RNA-seq) and from RNA
interference to gene knockouts using Clustered Regularly Interspaced Short Palindromic …

Intracellular genomic transplant and methods of therapy

B Moriarity, B Webber, M Choudhry… - US Patent …, 2024 - Google Patents
2018-11-08 Assigned to THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE
SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES reassignment THE …